Product datasheet Anti-MYO19 antibody [EPR12551-17] ab174278 1 Image Overview Product name Anti-MYO19 antibody [EPR12551-17] Description Rabbit monoclonal [EPR12551-17] to MYO19 Tested applications WB Species reactivity Reacts with: Human Immunogen Recombinant fragment within Human MYO19. The exact sequence is proprietary. Database link: Q96H55 Positive control HeLa, A549, K562 and 293T cell lysates. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Storage buffer pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA, 49% PBS Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR12551-17 Isotype IgG Applications Our Abpromise guarantee covers the use of ab174278 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. 1 Application Abreviews WB Application notes Notes 1/1000 - 1/10000. Predicted molecular weight: 109 kDa. Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP. Target Function Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO19 is involved in mitochondrial motility. Tissue specificity Widely expressed in multiple tissues and cell lines. Sequence similarities Contains 2 IQ domains. Contains 1 myosin head-like domain. Cellular localization Cytoplasm > cytoskeleton. Mitochondrion outer membrane. Probably associated with the outer membrane via its N-terminal mitochondrial targeting region. Anti-MYO19 antibody [EPR12551-17] images All lanes : Anti-MYO19 antibody [EPR1255117] (ab174278) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : A549 cell lysate Lane 3 : K562 cell lysate Lane 4 : 293T cell lysate Lysates/proteins at 10 µg per lane. Western blot - Anti-MYO19 [EPR12551-17] Predicted band size : 109 kDa antibody (ab174278) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. 2 Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3