Product datasheet
Anti-MYL9 antibody ab64161
1 Abreviews 1 References 2 Images
Overview
Product name
Anti-MYL9 antibody
Description
Rabbit polyclonal to MYL9
Tested applications
WB, IP, ELISA, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Bird, all Mammals
Immunogen
Synthetic peptide:
KKRPQRATSNVFAMFD
, corresponding to amino acids 12-27 of Human MYL9
Run BLAST with
Positive control
Run BLAST with
Colon, Smooth Muscle tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab64161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
IP
Use at an assay dependent concentration.
ELISA
1/5000 - 1/20000.
IHC-P
Use a concentration of 2.5 µg/ml.
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Target
Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and
nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor
capping, and cell locomotion.
Tissue specificity
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Sequence similarities
Contains 3 EF-hand domains.
Post-translational
modifications
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the
contractile activity. It is required to generate the driving force in the migration of the cells but not
necessary for localization of myosin-2 at the leading edge.
Anti-MYL9 antibody images
ab64161 at 2.5µg/ml staining MYL9 in Colon,
Smooth Muscle by Immunohistochemistry,
Formalin-fixed, paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - MYL9 antibody
(ab64161)
Anti-MYL9 antibody (ab64161) at 1/2000
dilution + Recombinant Human MYL9 protein
(ab104014) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Anti-MYL9 antibody (ab64161)
Exposure time : 4 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
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