Product datasheet
Recombinant human FKBP52 protein ab84189
2 Images
Overview
Product name
Recombinant human FKBP52 protein
Protein length
Full length protein
Description
Nature
Recombinant
Source
E. coli
Amino Acid Sequence
Species
Human
Sequence
MGSSHHHHHH SSGLVPRGSH MTAEEMKATE
SGAQSAPLPM EGVDISPKQD EGVLKVIKRE
GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS
LDRKDKFSFD LGKGEVIKAW DIAIATMKVG
EVCHITCKPE YAYGSAGSPP KIPPNATLVF
EVELFEFKGE DLTEEEDGGI IRRIQTRGEG
YAKPNEGAIV EVALEGYYKD KLFDQRELRF
EIGEGENLDL PYGLERAIQR MEKGEHSIVY
LKPSYAFGSV GKEKFQIPPN AELKYELHLK
SFEKAKESWE MNSEEKLEQS TIVKERGTVY
FKEGKYKQAL LQYKKIVSWL EYESSFSNEE
AQKAQALRLA SHLNLAMCHL KLQAFSAAIE
SCNKALELDS NNEKGLFRRG EAHLAVNDFE
LARADFQKVL QLYPNNKAAK TQLAVCQQRI
RRQLAREKKL YANMFERLAE EENKAKAEAS
SGDHPTDTEM KEEQKSNTAG SQSQVETEA
Specifications
Our Abpromise guarantee covers the use of ab84189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activity
Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1
µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH 8.0 using chymotrypsin.
Activity Assay
Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The
final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
Add 10 µl of recombinant FKBP4 protein with 1 µg in assay buffer.
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Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant
using a spectrophotometer.
Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that
contained 460mM LiCl to a concentration of 3 mM)
Record the increase in A405 nm for 30 minutes at 25°C.
Applications
SDS-PAGE
Functional Studies
Western blot
Endotoxin level
< 1.000 Eu/µg
Mass spectrometry
MALDI-TOF
Purity
> 90 % by SDS-PAGE.
ab84189 is purified using conventional chromatography techniques. Endotoxin Level: < 1.0 EU
per 1 µg of protein (determined by LAL method).
Form
Liquid
Additional notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Preservative: None
Constituents: 10% Glycerol, 20mM Tris, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Function
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of
unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90
(HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid
hormone receptors between cytoplasm and nuclear compartments (By similarity). The
isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening.
Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote
microtubule assembly.
Tissue specificity
Widely expressed.
Sequence similarities
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats.
Domain
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and
400.
Post-translational
modifications
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated
upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
Recombinant human FKBP52 protein images
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15% SDS-PAGE showing ab84189 at
approximately 53.9kDa (3µg).
SDS-PAGE - FKBP52 protein (His tag) (ab84189)
Anti-FKBP52 antibody (ab97306) at 1/3000
dilution + Recombinant human FKBP52
protein (ab84189) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot
Exposure time : 30 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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