Product datasheet
Anti-LIM kinase 2 antibody ab70116
3 Images
Overview
Product name
Anti-LIM kinase 2 antibody
Description
Rabbit polyclonal to LIM kinase 2
Specificity
ab70116 detects endogenous levels of total LIM kinase 2 protein.
Tested applications
WB, ELISA, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide derived from an internal sequence of human LIM kinase 2
Positive control
HeLa cells, HepG2 and HUVEC cell extracts and human heart tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Purification notes
ab70116 was affinity-purified from rabbit antiserum by affinity-chromatography using epitopespecific immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab70116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular
weight: 72 kDa).
ELISA
1/40000.
IHC-P
1/50 - 1/100.
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Application
Abreviews
ICC/IF
Notes
1/500 - 1/1000.
Target
Function
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in
vitro.
Tissue specificity
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a
is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain,
kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is
nearly equally observed.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated on serine and/or threonine residues by ROCK1.
Cellular localization
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and
Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely
translocated to the nucleus.
Anti-LIM kinase 2 antibody images
ab70116 at 1/500 dilution staining LIM kinase
2 in HeLa cells by Immunofluorescence, in the
absence or presence of the immunising
peptide.
Immunocytochemistry/ Immunofluorescence - LIM
kinase 2 antibody (ab70116)
ab70116 at 1/50 dilution staining LIM kinase
2 in human heart by Immunohistochemistry,
Paraffin-embedded tissue, in the absence or
presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - LIM kinase 2
antibody (ab70116)
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All lanes : Anti-LIM kinase 2 antibody
(ab70116) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : HUVEC cell extracts
Lane 3 : HepG2 cell extracts with immunising
peptide at 10 µg
Western blot - LIM kinase 2 antibody (ab70116)
Lysates/proteins at 30 µg per lane.
Predicted band size : 72 kDa
Observed band size : 72 kDa
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