Product datasheet
Anti-beta Actin (phospho Y55 + Y53) antibody ab59381
1 Abreviews 1 Image
Overview
Product name
Anti-beta Actin (phospho Y55 + Y53) antibody
Description
Rabbit polyclonal to beta Actin (phospho Y55 + Y53)
Specificity
This antibody detects endogenous levels of beta Actin only when phosphorylated at tyrosine
53/55.
Tested applications
ELISA, IHC-P, WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
Immunogen
Synthesized phosphopeptide derived from human beta Actin around the phosphorylation site of
tyrosine 53/55.
Positive control
Human skeletal muscle tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by
chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab59381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
1/5000.
IHC-P
Use at an assay dependent concentration.
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Application
Abreviews
WB
Notes
Use at an assay dependent concentration.
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are
ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of
dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle
contraction, often leading to abnormal postures. DYTJ patients manifest progressive,
generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing
loss.
Sequence similarities
Belongs to the actin family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated
mRNAs.
Anti-beta Actin (phospho Y55 + Y53) antibody images
Immunohistochemistry analysis of paraffinembedded human skeletal muscle tissue
using ab59381 at a 1/50 dilution.
Left image un-treated.
Right image treated with phosphopeptide.
Immunohistochemistry (Paraffin-embedded
sections) - beta Actin (phospho Y55 + Y53)
antibody (ab59381)
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Terms and conditions
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