Product datasheet
Anti-ARP2/3 subunit 1B antibody ab115217
2 Images
Overview
Product name
Anti-ARP2/3 subunit 1B antibody
Description
Goat polyclonal to ARP2/3 subunit 1B
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish
Immunogen
Synthetic peptide (
AYHSFLVEPISCH
) from N terminus amino acids 2-14 of ARP2/3 subunit 1B (Human)
Run BLAST with
Positive control
Run BLAST with
Human Thymus tissue, Human liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab115217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 41 kDa.
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
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Target
Function
Functions as component of the Arp2/3 complex which is involved in regulation of actin
polymerization and together with an activating nucleation-promoting factor (NPF) mediates the
formation of branched actin networks.
Sequence similarities
Belongs to the WD repeat ARPC1 family.
Contains 6 WD repeats.
Cellular localization
Cytoplasm > cytoskeleton.
Anti-ARP2/3 subunit 1B antibody images
Human thymus (formalin fixed, paraffin
embedded) stained with ab115217 at 2.5
ug/ml followed by biotinylated anti Goat IgG
secondary antibody, alkaline phosphatase
streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ARP2/3
subunit 1B antibody (ab115217)
Anti-ARP2/3 subunit 1B antibody (ab115217)
at 2 µg/ml + Human Liver lysate in RIPA buffer
at 35 µg
Predicted band size : 41 kDa
Western blot - Anti-ARP2/3 subunit 1B antibody
(ab115217)
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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