Product datasheet
Anti-Plectin antibody ab83497
2 References 2 Images
Overview
Product name
Anti-Plectin antibody
Description
Rabbit polyclonal to Plectin
Tested applications
WB, IP, ELISA, IHC-P, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to the C terminal of Human Plectin.
Positive control
Mouse and rat brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: PBS containing antibody stabilizer, pH 7.2
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab83497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 500 kDa
(predicted molecular weight: 531 kDa).
IP
Use a concentration of 2 - 5 µg/ml.
ELISA
Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
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Target
Function
Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate
filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin
to membrane complexes in muscle. May be involved not only in the filaments network, but also in
the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in
the maintenance of myofibers integrity.
Tissue specificity
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
Involvement in disease
Epidermolysis bullosa simplex with pyloric atresia
Epidermolysis bullosa simplex, with muscular dystrophy
Epidermolysis bullosa simplex, Ogna type
Limb-girdle muscular dystrophy 2Q
Sequence similarities
Belongs to the plakin or cytolinker family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 33 plectin repeats.
Contains 4 spectrin repeats.
Domain
The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins,
lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
Post-translational
modifications
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
Anti-Plectin antibody images
All lanes : Anti-Plectin antibody (ab83497) at
1/500 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Predicted band size : 531 kDa
Observed band size : 500 kDa
Western blot - Prostate Specific Antigen antibody
(ab83497)
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ICC/IF image of ab83497 stained PC12 cells.
The cells were 4% formaldehyde fixed (10
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab83497, 5µg/ml) overnight
at +4°C. The secondary antibody (green) was
Alexa Fluor® 488 goat anti-rabbit IgG (H+L)
Immunocytochemistry/ ImmunofluorescencePlectin antibody(ab83497)
used at a 1/1000 dilution for 1h. Alexa Fluor®
594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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