Product datasheet
Anti-Keratin antibody [C-11] ab118817
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Overview
Product name
Anti-Keratin antibody [C-11]
Description
Mouse monoclonal [C-11] to Keratin
Specificity
Reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
Tested applications
WB, IP, IHC-P, ICC, Flow Cyt
Species reactivity
Reacts with: Human
Predicted to work with: all Mammals
Immunogen
Raised against keratin-enriched preparation from Human epidermoid carcinoma cell line A431.
Positive control
Human Breast tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Purity
Protein A purified
Clonality
Monoclonal
Clone number
C-11
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab118817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IP
Use at an assay dependent concentration.
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Application
Abreviews
IHC-P
Notes
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is
suitable for use as an isotype control with this antibody.
Target
Tissue specificity
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus,
papillae of tongue and hair follicle outer root sheath.
Involvement in disease
Defects in KRT6A are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also
known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal
dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening
and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and
oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
Sequence similarities
Belongs to the intermediate filament family.
Anti-Keratin antibody [C-11] images
ab118817, at 10 µg/ml, staining Keratin in
formalin-fixed, paraffin-embedded Human
Breast tissue by Immunohistochemistry using
a biotinylated anti-mouse IgG secondary
antibody, alkaline phosphatase-streptavidin
and chromogen.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Keratin
antibody [C-11] (ab118817)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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