Product datasheet
Anti-Claudin 6 antibody ab107059
2 Abreviews 4 Images
Overview
Product name
Anti-Claudin 6 antibody
Description
Rabbit polyclonal to Claudin 6
Tested applications
WB, ICC/IF, IHC-P
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide between amino acids 133-163 from the central region of Human Claudin 6
(NP_067018.2), conjugated to KLH.
Positive control
Mouse heart tissue lysate This antibody gave a positive result in IHC in the following FFPE
tissue: Human normal kidney.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Purification notes
ab107059 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab107059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/100 - 1/500. Predicted molecular weight: 23 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 5 µg/ml.
Target
1
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Sequence similarities
Belongs to the claudin family.
Cellular localization
Cell junction > tight junction. Cell membrane.
Anti-Claudin 6 antibody images
Anti-Claudin 6 antibody (ab107059) at 1/100
dilution + Mouse heart tissue lysate at 35 µg
Predicted band size : 23 kDa
Western blot - Claudin 6 antibody (ab107059)
ab107059 staining Claudin 6 in murine
AGDC 5 chondocytic cells by
Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldhyde and
permeabilized in 0.1% Triton X-100 prior to
blocking in 10% FBS in PBS for 2 hours at
room temperature. The primary antibody was
diluted 1/50 and incubated with the sample for
12 hours at 4°C with overnight gyratory
incubation. The secondary antibody was
Immunocytochemistry/ Immunofluorescence -
Alexa Fluor® 594-conjugated goat anti-
Claudin 6 antibody (ab107059)
Rabbit polyclonal, diluted 1/50.
This image is courtesy of Dr Gerald Davies.
Red staining is Claudin 6 and the blue is
nuclear counterstaining with DAPI. Oil
immersion microscopy was at 100X using an
Olympus BX21 fluorescent microscope with
an Infinity 3 camera.
2
All lanes : Anti-Claudin 6 antibody
(ab107059) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from
mouse frontal cortex.
Lane 2 : Whole tissue lysate prepared from
Western blot - Claudin 6 antibody (ab107059)
mouse temporal cortex.
Lane 3 : Whole tissue lysate prepared from
mouse occipital cortex.
Lane 4 : Whole tissue lysate prepared from
mouse cerebellum.
Lane 5 : Whole tissue lysate prepared from
mouse brainstem.
Lane 6 : Whole tissue lysate prepared from
mouse muscle.
Lane 7 : Whole tissue lysate prepared from
mouse liver.
Lane 8 : Whole tissue lysate prepared from
mouse lung.
Lysates/proteins at 24 µg per lane.
Secondary
Goat anti-rabbit IgG conjugated to HRP at
1/10000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 23 kDa
Exposure time : 10 seconds
Image courtesy of Dr Gerald Davies by
Abreview.
3
IHC image of Claudin 6 staining in Human
normal kidney formalin fixed paraffin
embedded tissue section, performed on a
Leica BondTM system using the standard
protocol F. The section was pre-treated using
heat mediated antigen retrieval with sodium
citrate buffer (pH6, epitope retrieval solution
1) for 20 mins. The section was then
incubated with ab107059, 5µg/ml, for 15 mins
Immunohistochemistry (Formalin/PFA-fixed
at room temperature and detected using an
paraffin-embedded sections) - Anti-Claudin 6
HRP conjugated compact polymer system.
antibody (ab107059)
DAB was used as the chromogen. The
section was then counterstained with
haematoxylin and mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4