Product datasheet
Anti-Claudin 5 antibody ab15106
4 Abreviews 3 References 5 Images
Overview
Product name
Anti-Claudin 5 antibody
Description
Rabbit polyclonal to Claudin 5
Tested applications
ICC/IF, IHC-Fr, WB, IHC-P
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
Positive control
Lung
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab15106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 5 µg/ml.
IHC-Fr
Use at an assay dependent concentration. PubMed: 22547149
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P
1/50.
Target
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
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Sequence similarities
Belongs to the claudin family.
Cellular localization
Cell junction > tight junction. Cell membrane.
Anti-Claudin 5 antibody images
ab15106 staining Claudin 5 in Mouse lung
tissue sections by Immunohistochemistry
(IHC-P - paraformaldehyde-fixed, paraffinembedded sections). Tissue was fixed with
paraformaldehyde and blocked with 1.5%
serum for 1 hour at 37°C; antigen retrieval
was by heat mediation in a acetic acid.
Samples were incubated with primary
antibody (1/200 in blocking buffer) for 18
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Claudin 5
antibody (ab15106)
hours at 4°C. A Biotin-conjugated Goat antirabbit IgG polyclonal (1/200) was used as the
secondary antibody.
Image is courtesy of an anonymous AbReview
ICC/IF image of ab15106 stained MCF7
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab15106, 5µg/ml) overnight
at +4°C. The secondary antibody (green) was
Immunocytochemistry/ Immunofluorescence-
ab96899, DyLight® 488 goat anti-rabbit IgG
Claudin 5 antibody(ab15106)
(H+L) used at a 1/250 dilution for 1h. Alexa
Fluor® 594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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ab15106 staining Claudin 5 in human lung by
Immunohistochemistry (FFPE-sections).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Claudin 5 antibody
(ab15106)
ab15106 staining Claudin 5 in the Human
HBMEC cells by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde,
permeabilized with 1% Triton in PBS and
blocked with 10% serum for 30 minutes at
22°C. Samples were incubated with primary
antibody (1/200) for 16 hours at 4°C. An
Alexa Fluor® 488-conjugated Goat antirabbit polyclonal (1/500) was used as the
secondary antibody.
Immunocytochemistry/ Immunofluorescence Anti-Claudin 5 antibody (ab15106)
Image is courtesy of an anonymous AbReview
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Anti-Claudin 5 antibody (ab15106) at 1/1000
dilution + Mouse lung tissue lysate at 30 µg
Secondary
HRP-conjugated goat anti-rabbit IgG
polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 24 kDa
Western blot - Anti-Claudin 5 antibody (ab15106)
Observed band size : 22 kDa
This image is courtesy of an anonymous Abreview
Additional bands at : 35 kDa (possible nonspecific binding).
Exposure time : 1 minute
This image is courtesy of an anonymous
Abreview
Blocked with 5% milk for 1 hour at 25°C.
Incubated for 15 hours at 4°C with the primary
antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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