Product datasheet
Anti-Annexin A11 antibody ab166846
3 Images
Overview
Product name
Anti-Annexin A11 antibody
Description
Mouse polyclonal to Annexin A11
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Full length protein, corresponding to amino acids 1-505 of Human Annexin A11 (NP_001148.1).
Run BLAST with
Positive control
Run BLAST with
Human pancreas tissue lysate; Annexin A11 transfected 293T cell lysate; HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
pH: 7.20
Constituent: 99% PBS
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab166846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Target
Function
Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for
midbody formation and completion of the terminal phase of cytokinesis.
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Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats.
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Cellular localization
Cytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton
> spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates
around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes
relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic
pool. Localization to the nuclear envelope is cell-cycle dependent.
Anti-Annexin A11 antibody images
Immunofluorescent analysis of HeLa cells,
labeling Annexin A11 with ab166846 at
10µg/ml.
Immunocytochemistry/ Immunofluorescence Anti-Annexin A11 antibody (ab166846)
Anti-Annexin A11 antibody (ab166846) at 1
µg/ml + Human Pancreas tissue lysate at 50
µg
Secondary
Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size : 54 kDa
Western blot - Anti-Annexin A11 antibody
(ab166846)
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All lanes : Anti-Annexin A11 antibody
(ab166846) at 1 µg/ml
Lane 1 : Annexin A11 transfected 293T cell
lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Western blot - Anti-Annexin A11 antibody
(ab166846)
Secondary
Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size : 54 kDa
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