Overview
Product name
Description
Specificity
Tested applications
Species reactivity
Immunogen
Positive control
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Clone number
Isotype
Anti-Desmocollin 2 + 3 antibody [3G130]
Mouse monoclonal [3G130] to Desmocollin 2 + 3 ab69406 reacts with Desmocollin 2 and Desmocollin 3 human proteins, with a particularly strong reaction to Desmocollin 2. Crossreactivity with other desmocollin family members has not been observed.
WB, IP, ICC/IF
Reacts with: Human
Human Desmocollin 2 extracellular domain
A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Protein A purified
Monoclonal
3G130
IgG1
Applications
Our Abpromise guarantee covers the use of ab69406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
IP
ICC/IF
Application notes ICC/IF: Use at a concentration of 20 - 50 µg/ml.
1

IP: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 120 kDa, corresponding to Desmocollin 2, and a band of approximately 105 kDa, corresponding to
Desmocollin 3 (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Cellular localization
Members of the desmocollin subfamily are calcium-dependent glycoproteins. They are part of the cadherin superfamily. These desmosomal family members, together with the desmogleins, are mainly found in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction. They are required for cell adhesion and desmosome formation.
The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650kb combined.
Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
2