Product datasheet
Anti-Nck 1/2 antibody [AF6D7] ab80620
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Overview
Product name
Anti-Nck 1/2 antibody [AF6D7]
Description
Mouse monoclonal [AF6D7] to Nck 1/2
Specificity
ab80620 is specific for both Nck 1 and Nck 2
Tested applications
WB, IP
Species reactivity
Reacts with: Rat, Human
Immunogen
His-tagged recombinant full length Human NCK2, purified from E.coli
Positive control
Lysates from HeLa, U937, A431, SK-N-MC, Ramos or PC12 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Purity
Ammonium Sulphate Precipitation
Clonality
Monoclonal
Clone number
AF6D7
Isotype
IgG2a
Light chain type
lambda
Applications
Our Abpromise guarantee covers the use of ab80620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
IP
Application notes
IP: Use 0.5µl.
WB: 1/1000. Predicted molecular weight: 47 kDa.
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
NCK1 is one of the adaptor proteins which mediate specific protein-protein interactions in
signaling processes. Adaptor proteins usually contain several domains like SH2 and SH3 which
allow specific interactions with other specific proteins. NCK1 and NCK2 show high sequence
identity (68%) and have three SH3 domains and a C-terminal SH2 domain. Both of them bind
receptor tyrosine kinases such as PDGFR and other tyrosine phosphorylated proteins via their
SH2 domains. Various molecules which interact with SH domains of Nck and regulate signaling
process of actin cytoskeleton reorganization have been identified. Ncks are thought to have
important functions in the development of mesodermal structures during embryogenesis, linked
to a role in cell movement and cytoskeletal reorganization. Ncks also have a function in
modulating mRNA translation at the level of initiation by interacting eukayotic initiation factor 2
(eIF2). Under stressed conditions, protein synthesis is reduced by inhibiting the activity of eIF2
through phosphorylation, transiently inhibiting recycling of eIF2 into its active form.
Cellular localization
Cytoplasmic and Endoplasmic reticulum
Anti-Nck 1/2 antibody [AF6D7] images
All lanes : Anti-Nck 1/2 antibody [AF6D7]
(ab80620) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : SK-N-MC cell lysate
Lane 5 : Ramos cell lysate
Western blot - Nck 1/2 antibody [AF6D7]
Lane 6 : PC12 cell lysate
(ab80620)
Predicted band size : 47 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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