Product datasheet
Anti-EPS15 antibody ab84810
3 Images
Overview
Product name
Anti-EPS15 antibody
Description
Rabbit polyclonal to EPS15
Tested applications
IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Immunogen
Synthetic peptide corresponding to a region between residue 575 and 625 of human EPS15
(NP_001972.1).
Positive control
Human Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Ovarian
Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma and Testicular Seminoma tissues.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab84810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
Application notes
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer
will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Target
Relevance
The eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the
activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in
mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form.
Several physiological functions have been proposed for eps15. Phosphorylation by EGF and
PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and
endocytosis.
Cellular localization
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Recruited to the
plasma membrane upon EGFR activation and localizes to coated pits.
Anti-EPS15 antibody images
ab84810, at 1/100 dilution, staining EPS15 in
formalin-fixed, paraffin-embedded human
testicular seminoma by Immunohistochemistry
using a Red-fluorescent goat anti-rabbit IgG
highly cross-adsorbed Hilyte PlusTM 555
secondary antibody at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - EPS15 antibody
(ab84810)
ab84810, at 1/500 dilution, staining EPS15 in
formalin-fixed, paraffin-embedded human
testicular seminoma by Immunohistochemistry
using DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - EPS15 antibody
(ab84810)
ab84810, at 1/500 dilution, staining EPS15 in
formalin-fixed, paraffin-embedded human
prostate carcinoma by Immunohistochemistry
using DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - EPS15 antibody
(ab84810)
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