Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
Anti-DOK6 antibody
Rabbit polyclonal to DOK6
WB, ELISA, ICC/IF
Reacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal sequence within human DOK6.
COLO cell extracts. A549 cells.
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Isotype
Liquid
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Immunogen affinity purified
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab72730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular
ELISA
ICC/IF weight: 38 kDa).
1/10000.
1/500 - 1/1000.
Target
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Relevance DOK6 (docking protein 6) is a novel member of the DOK4/5 subclass of the p62 DOK family of intracellular adaptor molecules. DOK6 is highly expressed in the developing central nervous system and is co expressed with Ret in several locations, including sympathetic, sensory, and parasympathetic ganglia, as well as in the ureteric buds of the developing kidneys. Pull down assays using the DOK6 phosphotyrosine binding (PTB) domain and GDNF activated Ret indicate that DOK6 binds to the phosphorylated Ret Tyr(1062) residue. Moreover, ligand activation of Ret resulted in phosphorylation of tyrosine residue(s) located within the unique C terminus of DOK6 predominantly through a Src dependent mechanism, indicating that DOK6 is a substrate of the Ret Src signaling pathway.
Anti-DOK6 antibody images
Western blot - DOK6 antibody (ab72730)
All lanes : Anti-DOK6 antibody (ab72730) at
1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : COLO cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 38 kDa
Observed band size : 38 kDa ab72730 at 1/500 dilution staining DOK6 in
A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence -
DOK6 antibody (ab72730)
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Replacement or refund for products not performing as stated on the datasheet
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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