Product datasheet
Anti-14-3-3 eta antibody ab69591
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Overview
Product name
Anti-14-3-3 eta antibody
Description
Rabbit polyclonal to 14-3-3 eta
Specificity
Detects endogenous levels of total 14-3-3 eta protein.
Tested applications
WB, ELISA, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide (Human) from an internal region
Positive control
Jurkat cell extract HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab69591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular
weight: 28 kDa).
ELISA
1/5000.
ICC/IF
1/500 - 1/1000.
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Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized
signaling pathways. Binds to a large number of partners, usually by recognition of a
phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the
activity of the binding partner.
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels.
Sequence similarities
Belongs to the 14-3-3 family.
Anti-14-3-3 eta antibody images
All lanes : Anti-14-3-3 eta antibody
(ab69591) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with peptide at 10
µg
Lysates/proteins at 30 µg per lane.
Western blot - 14-3-3 eta antibody (ab69591)
Predicted band size : 28 kDa
Observed band size : 40 kDa
Additional bands at : 70 kDa,85 kDa. We
are unsure as to the identity of these extra
bands.
Immunofluorescence analysis of HeLa cells,
using ab69591 at 1/500-1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - 143-3 eta antibody (ab69591)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Terms and conditions
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