Product datasheet
Anti-14-3-3 eta antibody ab28665
1 Abreviews 2 References 3 Images
Overview
Product name
Anti-14-3-3 eta antibody
Description
Rabbit polyclonal to 14-3-3 eta
Specificity
The immunogen has 100% homology to human14-3-3 protein eta and 80% homology to 14-3-3
protein gamma. Cross-reactivity testing for this antibody has not been undertaken in the
laboratory.
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
Immunogen
Synthetic peptide, corresponding to a region between amino acids 71-120 of Human 14-3-3 eta
Positive control
WB: human fetal brain IHC: human skin and intestine
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab28665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 0.5 µg/ml. Detects a band of approximately 28 kDa
(predicted molecular weight: 28 kDa).Can be blocked with 14-3-3 eta peptide
(ab197887). Good results were obtained when blocked with 5% non-fat dry milk
in 0.05% PBS-T.
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Application
Abreviews
IHC-P
Notes
Use at an assay dependent concentration.
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized
signaling pathways. Binds to a large number of partners, usually by recognition of a
phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the
activity of the binding partner.
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels.
Sequence similarities
Belongs to the 14-3-3 family.
Anti-14-3-3 eta antibody images
Anti-14-3-3 eta antibody (ab28665) at 0.12
µg/ml + Human fetal brain lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000
dilution
developed using the ECL technique
Predicted band size : 28 kDa
Observed band size : 28 kDa
Western blot - 14-3-3 eta antibody (ab28665)
14-3-3 eta expression detected in paraffinembedded sections of human skin, using
ab28665 at 4-8ug/ml. The arrows indicate
positive staining of squamous epithelial cells.
Immunohistochemistry (Paraffin-embedded
sections) - 14-3-3 eta antibody (ab28665)
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14-3-3 eta expression detected in paraffinembedded sections of human intestine, using
ab28665 at 4-8ug/ml. The arrows indicate
positive staining of epithelial cells of intestinal
villi.
Immunohistochemistry (Paraffin-embedded
sections) - 14-3-3 eta antibody (ab28665)
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