Product datasheet
Anti-IRS2 antibody [EP903Y] ab46811
1 References 2 Images
Overview
Product name
Anti-IRS2 antibody [EP903Y]
Description
Rabbit monoclonal [EP903Y] to IRS2
Tested applications
WB, IHC-P, ICC/IF, IP
Species reactivity
Reacts with: Human
Immunogen
A synthetic peptide corresponding to residues near the N-terminus of human IRS2.
Positive control
293 cell lysate + insulin; human cervical carcinoma tissue.
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Purification notes
This antibody has not been purified. It is provided in tissue culture supernatant and storage
buffer.
Clonality
Monoclonal
Clone number
EP903Y
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab46811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
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Application
Abreviews
Notes
IHC-P
ICC/IF
IP
Application notes
ICC/IF: 1/100 - 1/250.
IHC-P: 1/50 - 1/100.
IP: 1/50.
WB: 1/100,000. Predicted molecular weight: 137 kDa.
Is unsuitable for Flow Cytometry.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May mediate the control of various cellular processes by insulin.
Sequence similarities
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytosol.
Anti-IRS2 antibody [EP903Y] images
Anti-IRS2 antibody [EP903Y] (ab46811) at
1/100000 dilution + 293 cell lysate at
10ug/lane, plus insulin
Secondary
Goat-anti rabbit, HRP labelled, at 1/2000
dilution
Predicted band size : 137 kDa
Observed band size : 160 kDa
Additional bands at : 50 kDa (possible nonWestern blot - IRS2 antibody [EP903Y] (ab46811)
specific binding).
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This image shows human cervical carcinoma
stained with ab46811 at 1/50 dilution.
Immunohistochemistry (Paraffin-embedded
sections) - IRS2 antibody [EP903Y] (ab46811)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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