Product datasheet
Anti-WASP antibody ab74904
4 Images
Overview
Product name
Anti-WASP antibody
Description
Rabbit polyclonal to WASP
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of
Human WASP .Read Abcam's proprietary immunogen policy(Peptide available as ab74903.)
Positive control
This antibody gave a positive signal in the following Whole Cell Lysates: Saos 2, LOVO, HT
1080, EBS E14 TG2A Day 0
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab74904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa
(predicted molecular weight: 53 kDa).
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 5 µg/ml.
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Target
Function
Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates
the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization.
Possible regulator of lymphocyte and platelet function.
Tissue specificity
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Involvement in disease
Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known
as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive
immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody
diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900].
Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood,
resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299].
XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections,
severe congenital neutropenia, and monocytopenia.
Sequence similarities
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain.
Domain
The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the
autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a
conformation change and leads to activation.
Cellular localization
Cytoplasm > cytoskeleton.
Anti-WASP antibody images
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All lanes : Anti-WASP antibody (ab74904) at
1 µg/ml
Lane 1 : Saos 2 (Human epithelial-like
osteosarcoma cell line) Whole Cell Lysate
Lane 2 : LOVO (Human colon
adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HT 1080 (Human fibrosarcoma)
Whole Cell Lysate
Lane 4 : EBS E14 TG2A Day 0 (Mouse
Pluripotent Embryonic Stem Cell) Whole Cell
Western blot - WASP antibody (ab74904)
Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 100 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 3 minutes
IHC image of WASP staining in human
Cerebral cortex FFPE section, performed on
a BondTM system using the standard protocol
F. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate
buffer (pH6, epitope retrieval solution 1) for 20
mins. The section was then incubated with
ab74904, 5µg/ml, for 15 mins at room
temperature and detected using an HRP
Immunohistochemistry (Formalin/PFA-fixed
conjugated compact polymer system. DAB
paraffin-embedded sections) - WASP antibody
was used as the chromogen. The section was
(ab74904)
then counterstained with haematoxylin and
mounted with DPX
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All lanes : Anti-WASP antibody (ab74904) at
1 µg/ml
Lane 1 : Saos-2
Lane 2 : LOVO (Human colon
adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HT 1080 (Human fibrosarcoma)
Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver
carcinoma cell line)
Western blot - WASP antibody (ab74904)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 100 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 20 minutes
ICC/IF image of ab74904 stained HepG2
cells. The cells were 4% PFA fixed (10 min)
and then incubated in 1%BSA / 10% normal
goat serum / 0.3M glycine in 0.1% PBSTween for 1h to permeabilise the cells and
block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab74904, 5µg/ml) overnight
at +4°C. The secondary antibody (green) was
Immunocytochemistry/ Immunofluorescence Anti-WASP antibody (ab74904)
ab96899 Dylight 488 goat anti-rabbit IgG
(H+L) used at a 1/250 dilution for 1h. Alexa
Fluor® 594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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