Product datasheet
Anti-PDLIM5 antibody ab196559
2 Images
Overview
Product name
Anti-PDLIM5 antibody
Description
Rabbit polyclonal to PDLIM5
Specificity
This antibody detects endogenous level of total PDLIM5 protein.
Tested applications
IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
Recombinant fragment corresponding to Human PDLIM5 (C terminal).
Database link: Q96HC4
Positive control
HL60, BT474, HepG2, MCF7 cell extracts. Mouse lung, heart, kidney and uterus tissue extracts.
U20S cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Purity
Immunogen affinity purified
Purification notes
Antibodies were purified by affinity purification using immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab196559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/50 - 1/200.
1
Application
Abreviews
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF
1/50 - 1/200.
Target
Function
May play an important role in the heart development by scaffolding PKC to the Z-disk region.
May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the
development of heart hypertrophy. Contributes to the regulation of dendritic spine
morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.
Tissue specificity
Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients
with bipolar disorder, schizophrenia, and major depression.
Sequence similarities
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cellular localization
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction >
synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites.
Anti-PDLIM5 antibody images
All lanes : Recombinant Human Superoxide
Dismutase 1 protein (ab159559) at 1/500
dilution
Lane 1 : HL60 extracts
Lane 2 : BT474 extracts
Lane 3 : HepG2 extracts
Western blot - Anti-PDLIM5 antibody (ab196559)
Lane 4 : MCF7 extracts
Lane 5 : Mouse lung extracts
Lane 6 : Mouse heart extracts
Lane 7 : Mouse kidney extracts
Lane 8 : Mouse uterus extracts
Predicted band size : 64 kDa
2
Immunofluorescence analysis of U20S cells
labeling PDLIM5 using ab196559, at 1/50
dilution. Lower panel shows DAPI for nuclear
staining (Blue).
Immunocytochemistry/ Immunofluorescence Anti-PDLIM5 antibody (ab196559)
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