Product datasheet
Anti-Clathrin light chain antibody [CON.1] ab24579
2 Abreviews 2 References 2 Images
Overview
Product name
Anti-Clathrin light chain antibody [CON.1]
Description
Mouse monoclonal [CON.1] to Clathrin light chain
Specificity
ab24579 recognises Clathrin Light Chain.
Tested applications
IHC-P, WB, ICC/IF, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: all Mammals
Immunogen
Synthetic peptide:
EEDPAAAFLAQQESEIAGIEND
, corresponding to amino acids 23/44 of Cow Clathrin light chain
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.03% Thimerosal (merthiolate)
Constituents: PBS
Purity
Protein A purified
Clonality
Monoclonal
Clone number
CON.1
Isotype
IgG2b
Applications
Our Abpromise guarantee covers the use of ab24579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/1000. (see Abreview).
WB
1/250. Predicted molecular weight: 25-29 kDa.
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Application
Abreviews
Notes
ICC/IF
Use at an assay dependent concentration.
IP
1/200.
Target
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and
vesicles.
Anti-Clathrin light chain antibody [CON.1] images
Formalin fixed HeLa cells double stained with
the monoclonal antibody to clathrin,
(ab24579, Red) and giantin (green). The
nucleus was visualized with DAPI stain.
Immunocytochemistry/ Immunofluorescence Anti-Clathrin light chain antibody [CON.1]
(ab24579)
ab74579 staining Clatherin light chain in
human prostate carcinoma tissue by
immunohistochemistry (IHC-P formaldehyde-fixed paraffin-embedded
sections). Tissue samples were fixed with
formaldehyde and blocked with 1% serum for
1 hour at 21 °C. Antigen retrieval was by heat
mediation in citrate buffer (pH6). Samples
were incubated with primary antibody 1/1000
Immunohistochemistry (Formalin/PFA-fixed
(PBS) for 1 hour at 21 °C. A Biotin-
paraffin-embedded sections) - Clathrin light chain
conjugated Donkey polyclonal to mouse IgG,
antibody [CON.1] (ab24579)
dilution 1/200, was used as secondary
This image is courtesy of an anonymous Abreview
antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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