Product datasheet
Anti-beta B1 Crystallin antibody ab54655
1 Abreviews 2 Images
Overview
Product name
Anti-beta B1 Crystallin antibody
Description
Mouse monoclonal to beta B1 Crystallin
Tested applications
WB
Species reactivity
Reacts with: Human, Recombinant Fragment
Immunogen
Recombinant full length protein, corresponding to amino acids 1-253 of Human beta B1
Crystallin
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab54655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as
immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Target
Function
Crystallins are the dominant structural components of the vertebrate eye lens.
Involvement in disease
Defects in CRYBB1 are the cause of cataract congenital nuclear autosomal recessive type 3
(CATCN3) [MIM:611544]. A congenital cataract affecting the central nucleus of the eye. Nuclear
cataracts are often not highly visually significant. The density of the opacities varies greatly from
fine dots to a dense, white and chalk-like, central cataract. The condition is usually bilateral.
Nuclear cataracts are often combined with opacified cortical fibers encircling the nuclear
opacity, which are referred to as cortical riders.
Defects in CRYBB1 are a cause of cataract-microcornea syndrome (CAMIS) [MIM:116150]. An
ocular disorder characterized by the association of congenital cataract and microcornea without
any other systemic anomaly or dysmorphism. Clinical findings include a corneal diameter inferior
to 10 mm in both meridians in an otherwise normal eye, and an inherited cataract, which is most
often bilateral posterior polar with opacification in the lens periphery. The cataract progresses to
form a total cataract after visual maturity has been achieved, requiring cataract extraction in the
first to third decade of life. Microcornea-cataract syndrome can be associated with other rare
ocular manifestations, including myopia, iris coloboma, sclerocornea and Peters anomaly.
Transmission is in most cases autosomal dominant, but cases of autosomal recessive
transmission have recently been described.
Sequence similarities
Belongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains.
Domain
Has a two-domain beta-structure, folded into four very similar Greek key motifs.
Post-translational
modifications
Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated
forms, leading to impaired oligomerization and protein insolubilization.
Anti-beta B1 Crystallin antibody images
Western blot against tagged recombinant
protein immunogen using ab54655 beta B1
Crystallin antibody at 1ug/ml. Predicted band
size of immunogen is 54 kDa.
This antibody has only been tested in WB
against the recombinant fragment used as
immunogen. We have no data on the
Western blot - beta B1 Crystallin antibody
detection of endogenous protein.
(ab54655)
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All lanes : Anti-beta B1 Crystallin antibody
(ab54655) at 1/100 dilution
Lane 1 : Zebrafish lens homogenate
Lane 2 : Zebrafish lens homogenate
Lane 4 : Human Beta B1 crystallin
Lane 5 : Human Beta B2 crystallin (Negative
Control)
Lysates/proteins at 1 µg per lane.
Western blot - beta B1 Crystallin antibody
Secondary
(ab54655)
Alkaline phosphatase-conjugated Goat antimouse IgG monoclonal at 1/3000 dilution
Observed band size : 45 kDa
Additional bands at : 30 kDa,82 kDa. We
are unsure as to the identity of these extra
bands.
This image is courtesy of an Abreview
submitted by Dr. Leonel Trujillo
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