Product datasheet
Anti-RDH12 antibody ab173449
1 Image
Overview
Product name
Anti-RDH12 antibody
Description
Rabbit polyclonal to RDH12
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Cow
Immunogen
Full length protein corresponding to Human RDH12 aa 1-316. (NP_689656.1).
Sequence:
MLVTLGLLTSFFSFLYMVAPSIRKFFAGGVCRTNVQLPGKVVVITGANTG
IGKETARELASRGARVYIACRDVLKGESAASEIRVDTKNSQVLVRKLDLS
DTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFETHLGVNHLG
HFLLTYLLLEQLKVSAPARVVNVSSVAHHIGKIPFHDLQSEKRYSRGFAY
CHSKLANVLFTRELAKRLQGTGVTTYAVHPGVVRSELVRHSSLLCLLWRL
FSPFVKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRTWVSPRARNNKTA
ERLWNVSCELLGIRWE
Database link: Q96NR8
Run BLAST with
Positive control
Run BLAST with
RDH12 transfected 293T cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.2
Constituent: 100% PBS
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab173449 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
Target
Function
Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPHdependent retinal reductase. Displays high activity toward 9-cis and all-trans-retinol. Also
involved in the metabolism of short-chain aldehydes. No steroid dehydrogenase activity
detected. Might be the key enzyme in the formation of 11-cis-retinal from 11-cis-retinol during
regeneration of the cone visual pigments.
Tissue specificity
Widely expressed, mostly in eye, kidney, brain, skeletal msucle and stomach.
Involvement in disease
Defects in RDH12 are the cause of Leber congenital amaurosis type 13 (LCA13) [MIM:612712].
LCA designates a clinically and genetically heterogeneous group of childhood retinal
degenerations, generally inherited in an autosomal recessive manner. Affected infants have little
or no retinal photoreceptor function as tested by electroretinography. LCA represents the most
common genetic cause of congenital visual impairment in infants and children.
Defects in RDH12 are the cause of retinitis pigmentosa type 53 (RP53) [MIM:612712]. RP53 is
a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is
characterized by retinal pigment deposits visible on fundus examination and primary loss of rod
photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have
night vision blindness and loss of midperipheral visual field. As their condition progresses, they
lose their far peripheral visual field and eventually central vision as well.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Anti-RDH12 antibody images
All lanes : Anti-RDH12 antibody (ab173449)
at 1 µg/ml
Lane 1 : RDH12 transfected 293T cell line
lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
Secondary
Western blot - Anti-RDH12 antibody (ab173449)
Goat Anti-Rabbit IgG (H+L), Peroxidase
Conjugated at 1/7500 dilution
developed using the ECL technique
Predicted band size : 35 kDa
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