Product datasheet Anti-CHST6 antibody ab84555 1 Image Overview Product name Anti-CHST6 antibody Description Rabbit polyclonal to CHST6 Tested applications WB Species reactivity Reacts with: Human Predicted to work with: Horse Immunogen Synthetic peptide corresponding to a region within internal sequence amino acids 346-395 (QELCAGALQL LGYRPVYSED EQRNLALDLV LPRGLNGFTW ASSTASHPRN) of human CHST6 (NP_067628). Run BLAST with Positive control Run BLAST with 293T cell lysate. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. Storage buffer Preservative: None Constituents: 2% Sucrose, PBS Purity Immunogen affinity purified Purification notes Purified by peptide affinity chromatography method. Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab84555 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. 1 Target Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. Tissue specificity Expressed in cornea. Mainly expressed in brain. Also expressed in spinal cord and trachea. Involvement in disease Defects in CHST6 are the cause of macular dystrophy, corneal, 1 (MCDC1) [MIM:217800]. An ocular disease characterized by bilateral, progressive corneal opacification, and reduced corneal sensitivity. Onset occurs in the first decade, usually between ages 5 and 9. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. The disease is due to deposition of an unsulfated keratan sulfate both within the intracellular space (within the keratocytes and endothelial cells) and in the extracellular corneal stroma. Macular corneal dystrophy is divided into the clinically indistinguishable types I, IA, and II based on analysis of the normally sulfated, or antigenic, keratan sulfate levels in serum and immunohistochemical evaluation of the cornea. Patients with types I and IA macular corneal dystrophy have undetectable serum levels of antigenic keratan sulfate, whereas those with type II macular corneal dystrophy have normal or low levels, depending on the population examined. Note=CHST6 homozygous missense mutations have been observed in patients with macular corneal dystrophy type I, while type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6. Sequence similarities Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. Cellular localization Golgi apparatus membrane. Anti-CHST6 antibody images Anti-CHST6 antibody (ab84555) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 44 kDa Observed band size : 44 kDa Western blot - CHST6 antibody (ab84555) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours 2 We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3