Product datasheet
Anti-CNG3 antibody ab97500
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Overview
Product name
Anti-CNG3 antibody
Description
Rabbit polyclonal to CNG3
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human
CNG3.Read Abcam's proprietary immunogen policy(Peptide available as ab109061.)
Positive control
This antibody gave a positive signal in Mouse Retina Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab97500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa
(predicted molecular weight: 79 kDa).
Target
Function
Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second
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messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation
channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering
channel gating, weakened the outward rectification in the presence of extracellular calcium,
increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when
coexpressed with CNGB3 (By similarity). Essential for the generation of light-evoked electrical
responses in the red-, green- and blue sensitive cones.
Tissue specificity
Prominently expressed in retina.
Involvement in disease
Defects in CNGA3 are the cause of achromatopsia type 2 (ACHM2) [MIM:216900]; also known
as total colorblindness or rod monochromacy (RMCH2). ACHM2 is an autosomal recessive
condition characterized by day blindness and photophobia. In ACHM2 patients the cones are
defective and the subjects see better at night.
Sequence similarities
Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily.
Contains 1 cyclic nucleotide-binding domain.
Cellular localization
Membrane.
Anti-CNG3 antibody images
Anti-CNG3 antibody (ab97500) at 1 µg/ml +
Mouse Retina Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 79 kDa
Western blot - CNG3 antibody (ab97500)
Observed band size : 79 kDa
Additional bands at : 46 kDa,55 kDa,70
kDa. We are unsure as to the identity of these
extra bands.
Exposure time : 2 minutes
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Terms and conditions
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