Product datasheet
Anti-Cadherin like 23 antibody ab81981
1 Image
Overview
Product name
Anti-Cadherin like 23 antibody
Description
Goat polyclonal to Cadherin like 23
Specificity
ab81981 is expected to recognize both reported isoforms (NP_071407.3; NP_443068.1).
Tested applications
WB, ELISA
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide:
C-YNISLYENVTVGTS
, corresponding to internal sequence amino acids 465-478 of Human Cadherin like 23
(NP_071407.3; NP_443068.1)
Run BLAST with
Positive control
Run BLAST with
Human Brain (Amygdala and Frontal Cortex) lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Purity
Immunogen affinity purified
Purification notes
ab81981 was purified from goat serum by ammonium sulphate precipitation followed by antigen
affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab81981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1
Application
Abreviews
Notes
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/1000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 59 kDa (predicted
molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Cadherin like 23 (or Cadherin 23) is, like other members of the cadherin family, a calciumdependent cell adhesion glycoprotein that preferentially interacts with itself in connecting cells. It
is a single pass type I membrane protein that contains 27 cadherin domains. Cadherin like 23 is
expressed in the neurosensory epithelium, where it is thought to be involved in stereocilia
organisation and hair bundle formation. Defects in the gene encoding Cadherin like 23 are a
cause of Usher syndrome, which is characterised by profound congenital sensorineural
deafness and eventual blindness. Mutations in this gene are also a cause of non-syndromic
sensorineural deafness autosomal recessive type 12 (DFNB12). Cadherin like 23 has also been
associated with age-related hearing loss.
Cellular localization
Cell membrane; Single-pass type I membrane protein
Anti-Cadherin like 23 antibody images
Anti-Cadherin like 23 antibody (ab81981) at 1
µg/ml + Human Amygdala lysate (in RIPA
buffer) at 35 µg
developed using the ECL technique
Predicted band size : 59 kDa
Observed band size : 59 kDa
Western blot - Cadherin like 23 antibody
(ab81981)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
2
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3