Product datasheet
Anti-Dopamine Transporter antibody ab111468
3 Abreviews 4 Images
Overview
Product name
Anti-Dopamine Transporter antibody
Description
Rabbit polyclonal to Dopamine Transporter
Tested applications
IHC-P, IP, ICC/IF, WB
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese
Hamster, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse
Dopamine Transporter.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in the following tissue lysates: Mouse Olfactory Bulb; Mouse
Forebrain; Rat Forebrain; Rat Cortex. IF/ICC: PC12 cell line.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab111468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
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Application
Abreviews
IHC-P
Notes
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
IP
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa
(predicted molecular weight: 69 kDa).
Target
Function
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent
reuptake into presynaptic terminals.
Involvement in disease
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It
is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia.
Other neurologic features include global developmental delay, bradikinesia and pyramidal tract
signs.
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
Cellular localization
Membrane.
Anti-Dopamine Transporter antibody images
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All lanes : Anti-Dopamine Transporter
antibody (ab111468) at 1 µg/ml
Lane 1 : Mouse Olfactory Bulb Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Rat Forebrain Rat Tissue Lysate
Lane 4 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-Dopamine Transporter
Goat Anti-Rabbit IgG H&L (HRP)
antibody (ab111468)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 69 kDa
Additional bands at : 47 kDa,62 kDa,77
kDa. We are unsure as to the identity of these
extra bands.
Exposure time : 1 minute
ab111468 staining mouse brain sections by
IHC-P. The tissue was fixed with
formaldehyde and a heat mediated antigen
retrival step was performed with citric acid pH
6. Blocking of the sample was done with 1%
BSA for 10 minutes at 21°C, followed by
staining with ab111468 at 1/500 in
TBS/BSA/azide for 2h at 21°C. A biotinylated
goat anti-rabbit polyclonal antibody at 1/200
was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Dopamine
Transporter antibody (ab111468)
This image is courtesy of an Abreview submitted by
Carl Hobbs
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Dopamine Transporter was
immunoprecipitated using 0.5mg Mouse
Brain tissue, 5µg of Rabbit polyclonal to
Dopamine Transporter and 50µl of protein G
magnetic beads (+). No antibody was added
to the control (-).
The antibody was incubated under agitation
with Protein G beads for 10min, Mouse Brain
tissue lysate lysate diluted in RIPA buffer was
Immunoprecipitation - Anti-Dopamine Transporter
antibody (ab111468)
added to each sample and incubated for a
further 10min under agitation.
Proteins were eluted by addition of 40µl SDS
loading buffer and incubated for 10min at
70oC; 10µl of each sample was separated on
a SDS PAGE gel, transferred to a
nitrocellulose membrane, blocked with 5%
BSA and probed with ab111468.
Secondary: Mouse monoclonal [SB62a]
Secondary Antibody to Rabbit IgG light chain
(HRP) (ab99697).
Band: 69kDa; Dopamine Transporter
ICC/IF image of ab111468 stained PC12
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab111468, 5µg/ml)
overnight at +4°C. The secondary antibody
Immunocytochemistry/ Immunofluorescence -
(green) was ab96899, DyLight® 488 goat
Anti-Dopamine Transporter antibody (ab111468)
anti-rabbit IgG (H+L) used at a 1/250 dilution
for 1h. Alexa Fluor® 594 WGA was used to
label plasma membranes (red) at a 1/200
dilution for 1h. DAPI was used to stain the cell
nuclei (blue) at a concentration of 1.43µM.
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