Product datasheet
Anti-SCAMP2 antibody [2F3] ab180385
2 Images
Overview
Product name
Anti-SCAMP2 antibody [2F3]
Description
Mouse monoclonal [2F3] to SCAMP2
Tested applications
ICC/IF, WB
Species reactivity
Reacts with: Human
Immunogen
Recombinant full length protein corresponding to Human SCAMP2 aa 1-329.
Sequence:
MSAFDTNPFADPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVP
VTQLPGSSQPAVLQPSVEPT
QPTPQAVVSAAQAGLLRQQEELDRKAAE
LERKERELQNTVANLHVRQNNWPPLPSWCPVKPCFYQDFSTE
IPADYQ
RICKMLYYLWMLHSVTLFLNLLACLAWFSGNSSKGVDFGLSILWFLIFTP
CAFLCWYRPIYKAF
RSDNSFSFFVFFFVFFCQIGIYIIQLVGIPGLGD
SGWIAALSTLDNHSLAISVIMMVVAGFFTLCAVLSV
FLLQRVHSLYRR
TGASFQQAQEEFSQGIFSSRTFHRAASSAAQGAFQGN
Database link: O15127
Run BLAST with
Positive control
Run BLAST with
HEK293T cells transfected with the pCMV6-ENTRY SCAMP2 cDNA; COS7 cells transiently
transfected by pCMV6-ENTRY SCAMP2 cDNA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Purity
Protein A purified
Purification notes
Purified from mouse ascites fluids by affinity chromatography.
Clonality
Monoclonal
1
Clone number
2F3
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab180385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/100.
WB
1/4000. Predicted molecular weight: 37 kDa.
Target
Function
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the SCAMP family.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane.
Anti-SCAMP2 antibody [2F3] images
All lanes : Anti-SCAMP2 antibody [2F3]
(ab180385) at 1/4000 dilution
Lane 1 : HEK293 cells transfected with
pCMV6-ENTRY control cDNA.
Lane 2 : HEK293 cells transfected with
pCMV6-ENTRY SCAMP2 cDNA.
Western blot - Anti-SCAMP2 [2F3] antibody
Lysates/proteins at 5 µg per lane.
(ab180385)
Predicted band size : 37 kDa
HEK293T cell lysates were generated from
transient transfection of the cDNA clone
(RC229105)
2
Immunofluorescence analysis of COS7 cells
transiently transfected by pCMV6-ENTRY
SCAMP2, labeling SCAMP2 with ab180385
at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence Anti-SCAMP2 [2F3] antibody (ab180385)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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