Product datasheet
Anti-SYT2 antibody ab113545
6 Abreviews 5 Images
Overview
Product name
Anti-SYT2 antibody
Description
Rabbit polyclonal to SYT2
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Mouse, Rat, Marmoset (common)
Predicted to work with: Dog, Human, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese
Hamster, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse SYT2.Read
Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord;
Cortex; Hippocampus; Cerebellum; Forebrain; Substantia Nigra. This antibody gave a positive
result in IHC in the following FFPE tissue: Mouse normal brain.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab113545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
IHC-P
Use a concentration of 0.5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa
(predicted molecular weight: 47 kDa).
Target
Function
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at
the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the
presence of both an acidic head group and a diacyl backbone.
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains.
Domain
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle >
secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
Anti-SYT2 antibody images
IHC-P image of SYT2 staining on sections
from rat thalamic nucleus (upper image) and
the CA3 region of the rat hippocampus (lower
image) using ab113545 (1:5000). The
sections were deparaffinized and subjected
to heat mediated antigen retrieval using citric
acid. The sections were blocked using 1%
BSA for 10 mins at 21°C. ab113545 was
diluted 1:5000 in TBS buffer (containing BSA
and Azide) and sections were then incubated
with ab113545 for 2 hours 10 minutes at
Immunohistochemistry (Formalin/PFA-fixed
21°C. The secondary antibody used was
paraffin-embedded sections) - Anti-SYT2 antibody
Biotin conjugated Goat polyclonal to Rabbit
(ab113545)
IgG (1:250).
Image courtesy of Carl Hobbs, Kings College
London, U.K.
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IHC-P image of SYT2 staining on sections
from mouse sagittal hippocampus (upper
image) and dentate nucleus of the
mouse cerebellum (lower image) using
ab113545 (1:5000). The sections were
deparaffinized and subjected to heat
mediated antigen retrieval using citric acid.
The sections were blocked using 1% BSA for
10 mins at 21°C. ab113545 was diluted
1:5000 in TBS buffer (containing BSA and
Azide) and sections were then incubated with
Immunohistochemistry (Formalin/PFA-fixed
ab113545 for 2 hours at 21°C. The secondary
paraffin-embedded sections) - Anti-SYT2 antibody
antibody used was Biotin conjugated Goat
(ab113545)
polyclonal to Rabbit IgG (1:250).
Image courtesy of Carl Hobbs, Kings College
London, U.K
IHC-P image of SYT2 staining on Marmoset
cerebellum sections using
ab113545(1:7000). The sections were
deparaffinized and subjected to heat
mediated antigen retrieval using citric acid.
The sections were blocked using 1% BSA for
10 mins at 21°C. ab113545 was diluted
1:7000 in TBS buffer (containing BSA and
Immunohistochemistry (Formalin/PFA-fixed
Azide) and sections were then incubated
paraffin-embedded sections) - Anti-SYT2 antibody
with ab113545 for 2 hours at 21°C. The
(ab113545)
secondary antibody used was Biotin
Image courtsey Carl Hobbs (King's College London,
United Kingdom)
conjugated Goat polyclonal to Rabbit IgG
(1:250).
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All lanes : Anti-SYT2 antibody (ab113545) at
1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Forebrain (Mouse) Tissue Lysate
Lane 7 : Mouse Substantia Nigra Tissue
Lysate
Western blot - Anti-SYT2 antibody (ab113545)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 70 kDa
Exposure time : 2 minutesThe predicted
molecular weight of SYT2 is 47 kDa
(SwissProt), however we expect to observe a
banding pattern around 70 kDa. Abcam
welcomes customer feedback and would
appreciate any comments regarding this
product and the data presented above.
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IHC image of SYT2 staining in Mouse normal
brain formalin fixed paraffin embedded tissue
section, performed on a Leica BondTM
system using the standard protocol F. The
section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer
(pH6, epitope retrieval solution 1) for 20 mins.
The section was then incubated with
ab113545, 0.5µg/ml, for 15 mins at room
Immunohistochemistry (Formalin/PFA-fixed
temperature and detected using an HRP
paraffin-embedded sections) - Anti-SYT2 antibody
conjugated compact polymer system. DAB
(ab113545)
was used as the chromogen. The section was
then counterstained with haematoxylin and
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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