Product datasheet
Anti-Syntaxin 4 antibody ab77037
2 References 5 Images
Overview
Product name
Anti-Syntaxin 4 antibody
Description
Mouse monoclonal to Syntaxin 4
Tested applications
WB, Flow Cyt, ICC/IF, ELISA
Species reactivity
Reacts with: Mouse, Human
Immunogen
Recombinant protein fragment with GST tag, corresponding to amino acids 19 - 120 of Human
Syntaxin 4 (NP_004595)
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab77037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 33 kDa.
Flow Cyt
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
an isotype control with this antibody.
ICC/IF
Use a concentration of 10 µg/ml.
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Application
Abreviews
Notes
ELISA
Use at an assay dependent concentration. Detection limit for ab77037 is
approximately 1ng/ml as a capture antibody.
Target
Function
Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the
translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with
STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing
vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of
synaptic vesicles at presynaptic active zones.
Tissue specificity
Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils
increases with differentiation.
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localization
Cell membrane.
Anti-Syntaxin 4 antibody images
Anti-Syntaxin 4 antibody (ab77037) at 1/500
dilution + HeLa cell lysate at 25 µg
Secondary
Goat anit-mouse IgG (H&L)-HRP conjugate at
1/2500 dilution
Predicted band size : 33 kDa
Western blot - Syntaxin 4 antibody (ab77037)
Observed band size : 34 kDa
Anti-Syntaxin 4 antibody (ab77037) at 1/500
dilution + recombinant protein Syntaxin 4 at
0.1 µg
Secondary
Goat anit-mouse IgG (H&L)-HRP conjugate at
1/2500 dilution
Western blot - Syntaxin 4 antibody (ab77037)
Predicted band size : 33 kDa
Observed band size : 37 kDa
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ab77037 tested in recombinant GST tagged
protein, with a detection limit of approximately
1 ng/ml
Sandwich ELISA - Syntaxin 4 antibody (ab77037)
ICC/IF image of ab77037 stained HeLa cells.
The cells were 100% methanol fixed (5 min)
and then incubated in 1%BSA / 10% normal
goat serum / 0.3M glycine in 0.1% PBSTween for 1h to permeabilise the cells and
block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab77037, 10µg/ml)
overnight at +4°C. The secondary antibody
(green) was Alexa Fluor® 488 goat antiImmunocytochemistry/ ImmunofluorescenceSyntaxin 4 antibody(ab77037)
mouse IgG (H+L) used at a 1/1000 dilution for
1h. Alexa Fluor® 594 WGA was used to label
plasma membranes (red) at a 1/200 dilution
for 1h. DAPI was used to stain the cell nuclei
(blue) at a concentration of 1.43µM.
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Overlay histogram showing HL60 cells
stained with ab77037 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry - Anti-Syntaxin 4 antibody
(ab77037, 1µg/1x106 cells) for 30 min at
(ab77037)
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in HL60
cells fixed in 4% paraformaldehyde (10
min)/permeabilized with 0.1% PBS-Tween for
20 min used under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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