Product datasheet
Anti-Kir4.1 antibody ab130707
3 Abreviews 2 Images
Overview
Product name
Anti-Kir4.1 antibody
Description
Rabbit polyclonal to Kir4.1
Tested applications
WB, IHC-Fr
Species reactivity
Reacts with: Human, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino
acids 202-230 of Human Kir4.1 (NP_002232.2; P78508).
Positive control
HL60 cell line lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Immunogen affinity purified
Purification notes
ab130707 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab130707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/100 - 1/500. Predicted molecular weight: 43 kDa.
IHC-Fr
1/100.
Target
Function
May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier
potassium channels are characterized by a greater tendency to allow potassium to flow into the
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cell rather than out of it. Their voltage dependence is regulated by the concentration of
extracellular potassium; as external potassium is raised, the voltage range of the channel
opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of
outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
Involvement in disease
Defects in KCNJ10 are the cause of seizures-sensorineural deafness-ataxia-mental retardationelectrolyte imbalance (SESAME) [MIM:612780]. A complex disorder characterized by
generalized seizures with onset in infancy, delayed psychomotor development, ataxia,
sensorineural hearing loss, hypokalemia, metabolic alkalosis, and hypomagnesemia.
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.
Cellular localization
Membrane.
Anti-Kir4.1 antibody images
ab130707 staining Kir4.1 in zebrafish retina
sections by IHC-Fr. The tissue was fixed with
paraformaldehyde and an antigen retrieval
step was performed with sodium citrate pH 6.
Blocking of the sample was done with 5%
BSA in PBS containing 01% Tween 20 and
0.5% Triton X, for 60 minutes at 23°C,
followed by staining with ab130707 at 1/100
in blocking solution for 16h at 4°C. An alexa
488 conjugated goat anti-rabbit polyclonal
antibody at 1/1000 was used as the
Immunohistochemistry (Frozen sections) - AntiKir4.1 antibody (ab130707)
This image is courtesy of an Abreview submitted by
Dr Ryan MacDonald
secondary antibody. Nuclei are stained in
blue with DAPI. Kir4.1 expression can be
observed in Muller cells (in green).
Anti-Kir4.1 antibody (ab130707) at 1/100
dilution + HL60 cell line lysates at 35 µg
developed using the ECL technique
Predicted band size : 43 kDa
Western blot - Anti-Kir4.1 antibody (ab130707)
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