Product datasheet
Anti-KCNQ1 antibody [S37A-10] ab84819
1 References 3 Images
Overview
Product name
Anti-KCNQ1 antibody [S37A-10]
Description
Mouse monoclonal [S37A-10] to KCNQ1
Tested applications
IP, Flow Cyt, IHC-P, IHC-Fr, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Fusion protein: AAASSPPRAE RKRWGWGRLP GARRGSAGLA KKCPFSLELA
EGGPAGGALY APIAPGAPGP APPASPAAPA APPVASDLGP RPPVSLDPRV
SIYSTRRPVL, corresponding to amino acids 2-101 of Human KCNQ1 (accession number:
P51787)
Run BLAST with
Positive control
Run BLAST with
Lysate from transfected COS-1 cells transiently expressing KCNQ1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Purity
Protein G purified
Clonality
Monoclonal
Clone number
S37A-10
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab84819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
an isotype control with this antibody.
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Application
Abreviews
Notes
IHC-P
Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr
Use a concentration of 0.1 - 1 µg/ml.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 75 kDa.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
Target
Function
Probably important in cardiac repolarization. Associates with KCNE1 (MinK) to form the I(Ks)
cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current.
Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current in CHO cells in
which cloned KCNQ1/KCNE1 channels were coexpressed with M1 muscarinic receptors. May
associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic
AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and
pathologically stimulated in cholera and other forms of secretory diarrhea.
Tissue specificity
Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood
leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.
Involvement in disease
Defects in KCNQ1 are the cause of long QT syndrome type 1 (LQT1) [MIM:192500]; also known
as Romano-Ward syndrome (RWS). Long QT syndromes are heart disorders characterized by a
prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope
and sudden death in response to exercise or emotional stress. LQT1 inheritance is an
autosomal dominant.
Defects in KCNQ1 are the cause of Jervell and Lange-Nielsen syndrome type 1 (JLNS1)
[MIM:220400]. JLNS1 is an autosomal recessive disorder characterized by congenital deafness,
prolongation of the QT interval, syncopal attacks due to ventricular arrhythmias, and a high risk of
sudden death.
Defects in KCNQ1 are the cause of atrial fibrillation familial type 3 (ATFB3) [MIM:607554]. Atrial
fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of
patients. It is characterized by disorganized atrial electrical activity and ineffective atrial
contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in
palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Defects in KCNQ1 are the cause of short QT syndrome type 2 (SQT2) [MIM:609621]. Short QT
syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT
interval on ECG in the absence of structural heart disease in affected individuals. They cause
syncope and sudden death.
Sequence similarities
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.1/KCNQ1 subsubfamily.
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively
charged amino acids at every third position.
Cellular localization
Cell membrane. Cytoplasmic vesicle membrane.
Form
There are 2 isoforms produced by alternative splicing. Isoform 2 also known as: TKvLQT1.
Anti-KCNQ1 antibody [S37A-10] images
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All lanes : Anti-KCNQ1 antibody [S37A-10]
(ab84819) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from CHO-T
cells transfected with mink-KvLQT1
Predicted band size : 75 kDa
Western blot - KCNQ1 antibody [S37A-10]
(ab84819)
ab84819 staining KCNQ1 in human
hippocampal tissue by IHC-P (Bouin's fixative
fixed paraffin embedded). The sample was
incubated with primary antibody at 100 and
with a fluorophore conjugated anti
mouse secondary at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - KCNQ1 antibody
[S37A-10] (ab84819)
Overlay histogram showing HEK293 cells
stained with ab84819 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
Flow Cytometry-Anti-KCNQ1 antibody [S37A-10]
(ab84819)
interactions followed by the antibody
(ab84819, 1µg/1x106 cells) for 30 min at
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
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