Product datasheet
Anti-Nav1.7 antibody [mAbcam62758] ab62758
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Overview
Product name
Anti-Nav1.7 antibody [mAbcam62758]
Description
Mouse monoclonal [mAbcam62758] to Nav1.7
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse
Nav1.7.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in Mouse and Rat Spinal Cord Tissue Lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mAbcam62758
Myeloma
Sp2/0-Ag14
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab62758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa
(predicted molecular weight: 226 kDa).
ICC/IF
Use a concentration of 5 - 10 µg/ml.
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Target
Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming
opened or closed conformations in response to the voltage difference across the membrane, the
protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with
their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in
pain mechanisms, especially in the development of inflammatory pain.
Tissue specificity
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth
muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the
central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root
ganglion.
Involvement in disease
Defects in SCN9A are the cause of primary erythermalgia (PERYTHM) [MIM:133020]. It is an
autosomal dominant disease characterized by recurrent episodes of severe pain associated
with redness and warmth in the feet or hands.
Defects in SCN9A are the cause of congenital indifference to pain autosomal recessive
(CIPAR) [MIM:243000]; also known as channelopathy-associated insensitivity to pain. A
disorder characterized by congenital inability to perceive any form of pain, in any part of the
body. All other sensory modalities are preserved and the peripheral and central nervous systems
are apparently intact. Patients perceive the sensations of touch, warm and cold temperature,
proprioception, tickle and pressure, but not painful stimuli. There is no evidence of a motor or
sensory neuropathy, either axonal or demyelinating.
Defects in SCN9A are a cause of paroxysmal extreme pain disorder (PEPD) [MIM:167400];
previously known as familial rectal pain (FRP). PEPD is an autosomal dominant paroxysmal
disorder of pain and autonomic dysfunction. The distinctive features are paroxysmal episodes of
burning pain in the rectal, ocular, and mandibular areas accompanied by autonomic
manifestations such as skin flushing.
Defects in SCN9A are a cause of generalized epilepsy with febrile seizures plus type 7
(GEFS+7) [MIM:604233]. GEFS+7 is a rare autosomal dominant, familial condition with
incomplete penetrance and large intrafamilial variability. Patients display febrile seizures
persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. This
disease combines febrile seizures, generalized seizures often precipitated by fever at age 6
years or more, and partial seizures, with a variable degree of severity.
Defects in SCN9A are the cause of familial febrile convulsions type 3B (FEB3B) [MIM:604403].
FEB3B consists of seizures associated with febrile episodes in childhood without any evidence
of intracranial infection or defined pathologic or traumatic cause. It is a common condition,
affecting 2-5% of children aged 3 months to 5 years. The majority are simple febrile seizures
(generally defined as generalized onset, single seizures with a duration of less than 30 minutes).
Complex febrile seizures are characterized by focal onset, duration greater than 30 minutes,
and/or more than one seizure in a 24 hour period. The likelihood of developing epilepsy following
simple febrile seizures is low. Complex febrile seizures are associated with a moderately
increased incidence of epilepsy.
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Contains 1 IQ domain.
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6)
and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and
are characterized by a series of positively charged amino acids at every third position.
Post-translational
modifications
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be
ubiquitinated by NEDD4.
Cellular localization
Membrane. In neurite terminals.
Anti-Nav1.7 antibody [mAbcam62758] images
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All lanes : Anti-Nav1.7 antibody
[mAbcam62758] (ab62758) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Mouse IgG H&L (HRP)
preadsorbed (ab97040) at 1/5000 dilution
Western blot - Nav1.7 antibody [mAbcam62758]
developed using the ECL technique
(ab62758)
Performed under reducing conditions.
Predicted band size : 226 kDa
Observed band size : 200 kDa
Additional bands at : 30 kDa,38 kDa,41
kDa,50 kDa. We are unsure as to the identity
of these extra bands.
Exposure time : 3 minutes
ICC/IF image of ab62758 stained PC12 cells.
The cells were 4% PFA fixed (10 min) and
then incubated in 1%BSA / 10% normal goat
serum / 0.3M glycine in 0.1% PBS-Tween for
1h to permeabilise the cells and block nonspecific protein-protein interactions. The cells
were then incubated with the antibody
(ab62758, 5µg/ml) overnight at +4°C. The
secondary antibody (green) was Alexa Fluor®
488 goat anti-mouse IgG (H+L) used at a
1/1000 dilution for 1h. Alexa Fluor® 594 WGA
Immunocytochemistry/ Immunofluorescence Nav1.7 antibody [mAbcam62758] (ab62758)
was used to label plasma membranes (red) at
a 1/200 dilution for 1h. DAPI was used to
stain the cell nuclei (blue) at a concentration of
1.43µM.
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