Product datasheet
Anti-5HT1B Receptor antibody ab13896
1 Abreviews 8 References 3 Images
Overview
Product name
Anti-5HT1B Receptor antibody
Description
Rabbit polyclonal to 5HT1B Receptor
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptides, corresponding to amino acids 8-26 (CAPPPPAGSETWVPQANLS) and
263-278 (STSSVTSINSRVPDVP) of 5HT1B Receptor.
Positive control
Tested with human brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Purity
Protein G purified
Purification notes
This antibody is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab13896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 40-41 kDa
(predicted molecular weight: 47 kDa).
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
IF
Use at an assay dependent concentration. PubMed: 24642693
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Target
Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic
hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this
receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Post-translational
modifications
Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
Palmitoylated.
Cellular localization
Cell membrane.
Anti-5HT1B Receptor antibody images
ab17976 staining 5HT1B in Human colon
tissue sections by Immunohistochemistry
(IHC-P - paraformaldehyde-fixed, paraffinembedded sections). Tissue was fixed with
10% NBFand blocked with antibody block for
10 minutes; antigen retrieval was by heat
mediation in CC1 Mild. Samples were
incubated with primary antibody (1/100
in antibody diluent) for 1 hour at 37°C. An
Immunohistochemistry (Formalin/PFA-fixed
undiluted Biotin-conjugated Goat anti-rabbit
paraffin-embedded sections) - Anti-5HT1B
IgG polyclonal was used as the secondary
Receptor antibody (ab13896)
antibody.
This image is courtesy of an anonymous Abreview
Predicted band size : 47 kDa
Western blot analysis of 5HT1B Receptor in
human brain lysate with ab13896. A protein
band of approximate molecular weight of 4041kDa was detected. Western blot analysis of
Western blot - Anti-5HT1B Receptor antibody
5HT1B Receptor in human brain lysate with
(ab13896)
ab13896. A protein band of approximate
molecular weight of 40-41kDa was detected.
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ab2607 (4µg/ml) staining 5HT1B in human
testis using an automated system (DAKO
Autostainer Plus). Using this protocol there is
strong staining of membrane compartment of
the mature seminiferous tubules and possible
interstitial cells.
Sections were rehydrated and antigen
retrieved with the Dako 3 in 1 AR buffer
EDTA pH 9.0 in a DAKO PT link. Slides were
Immunohistochemistry (Formalin/PFA-fixed
peroxidase blocked in 3% H2O2 in methanol
paraffin-embedded sections)-5HT1B Receptor
for 10 mins. They were then blocked with
antibody(ab13896)
Dako Protein block for 10 minutes (containing
casein 0.25% in PBS) then incubated with
primary antibody for 20 min and detected with
Dako envision flex amplification kit for 30
minutes. Colorimetric detection was
completed with Diaminobenzidine for 5
minutes. Slides were counterstained with
Haematoxylin and coverslipped under
DePeX. Please note that, for manual staining,
optimization of primary antibody
concentration and incubation time is
recommended. Signal amplification may be
required.
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