Product datasheet
Anti-KChIP2 antibody ab3473
1 Abreviews
Overview
Product name
Anti-KChIP2 antibody
Description
Rabbit polyclonal to KChIP2
Specificity
Detects KChIP 2 b and c from rat and rabbit samples as well as recombinant rat KChIP 2c. This
antibody does not detect KChIP 2a.
Tested applications
IHC-P, ICC, WB
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Dog, Non Human Primates
Predicted to work with: Human
Immunogen
Synthetic peptide corresponding to Human KChIP2 aa 6-24.
Sequence:
RKESLSDSRDLDGSYDQLT
(Peptide available as ab4991)
Run BLAST with
Run BLAST with
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Purity
Immunogen affinity purified
Primary antibody notes
Voltage-gated potassium (Kv) channels are key components of the potassium currents in the
heart and central nervous system. In the heart, Kv4 channels are involved in the repolarization
phase of the action potential. In the brain however, these channels prevent reverse-propagation
of action potentials. Found associated with Kv4 channels are a group of calcium-binding
proteins termed KChIPs (Kv channel interacting proteins). KChIPs are small molecular weight
proteins that bind to the cytoplasmic amino termini of Kv4 alpha-subunits and help to modulate
its function. There are currently three known KChIPs; KChIP 1 expressed in the brain; KChIP 2
expressed in the brain, heart, and lung, and has three isoforms, a, b, and c; and KChIP 3, also
known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator)
which is expressed in the brain and testis.
Clonality
Polyclonal
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab3473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 2 µg/ml. Can be blocked with KChIP2 peptide (ab4991).
By Western blot, this antibody detects ~33 and 26 kDa proteins representing
KChIP2b and c, respectively, from rat ventricular myocyte whole cell lysate.
Target
Function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium
channels. Probably modulates channels density, inactivation kinetics and rate of recovery from
inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates
KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell
surface.
Tissue specificity
Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and
hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein
endothelial cells. Not expressed in fetal heart.
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains.
Post-translational
modifications
Palmitoylated. Palmitoylation enhances association with the plasma membrane.
Cellular localization
Cell membrane.
Form
There are 9 isoforms produced by alternative splicing.
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Terms and conditions
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