Product datasheet
Anti-SCN3A antibody ab65164
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Overview
Product name
Anti-SCN3A antibody
Description
Rabbit polyclonal to SCN3A
Tested applications
ICC/IF, WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide derived from N terminal of human SCN3A.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab65164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/1000.
WB
1/1000 - 1/5000. Predicted molecular weight: 226 kDa.
Target
Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming
opened or closed conformations in response to the voltage difference across the membrane, the
protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with
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their electrochemical gradient.
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.3/SCN3A subfamily.
Contains 1 IQ domain.
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6)
and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and
are characterized by a series of positively charged amino acids at every third position.
Post-translational
modifications
May be ubiquitinated by NEDD4L; which would promote its endocytosis.
Phosphorylation at Ser-1501 by PKC in a highly conserved cytoplasmic loop slows inactivation
of the sodium channel and reduces peak sodium currents.
Cellular localization
Membrane.
Anti-SCN3A antibody images
ICC/IF image of ab65164 stained SHSY5Y
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab65164, 1/1000 dilution)
overnight at +4°C. The secondary antibody
(green) was Alexa Fluor® 488 goat anti-rabbit
IgG (H+L) used at a 1/1000 dilution for 1h.
Immunocytochemistry/ Immunofluorescence -
Alexa Fluor® 594 WGA was used to label
SCN3A antibody (ab65164)
plasma membranes (red) at a 1/200 dilution
for 1h. DAPI was used to stain the cell nuclei
(blue) at a concentration of 1.43µM.
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