Product datasheet
Anti-SCN1B antibody ab107370
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Overview
Product name
Anti-SCN1B antibody
Description
Rabbit polyclonal to SCN1B
Tested applications
ICC/IF, WB, IHC-P
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 70-98 of Human
SCN1B (NP_950238.1, NP_001028.1).
Positive control
Mouse Neuro-2a cell line lysates and Human brain tissue. This antibody gave a positive result
when used in the following formaldehyde fixed cell lines: HepG2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Purification notes
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab107370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/100 - 1/500. Predicted molecular weight: 25 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
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Target
Function
Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of
the sodium channel. The subunit beta-1 can modulate multiple alpha subunit isoforms from brain,
skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to
the nodes of Ranvier of developing axons and retain these channels at the nodes in mature
myelinated axons.
Tissue specificity
Abundantly expressed in skeletal muscle, heart and brain.
Involvement in disease
Defects in SCN1B are the cause of generalized epilepsy with febrile seizures plus type 1
(GEFS+1) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare
autosomal dominant, familial condition with incomplete penetrance and large intrafamilial
variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years
and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures,
generalized seizures often precipitated by fever at age 6 years or more, and partial seizures,
with a variable degree of severity.
Defects in SCN1B are the cause of Brugada syndrome type 5 (BRS5) [MIM:612838]. A
tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an
electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented
from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation),
the individual will faint and may die in a few minutes if the heart is not reset.
Sequence similarities
Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Cellular localization
Membrane.
Anti-SCN1B antibody images
ICC/IF image of ab107370 stained HepG2
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab107370 at 1µg/ml
overnight at +4°C. The secondary antibody
Immunocytochemistry/ Immunofluorescence Anti-SCN1B antibody (ab107370)
(green) was DyLight® 488 goat anti- rabbit
(ab96899) IgG (H+L) used at a 1/250 dilution
for 1h. Alexa Fluor® 594 WGA was used to
label plasma membranes (red) at a 1/200
dilution for 1h. DAPI was used to stain the cell
nuclei (blue) at a concentration of 1.43µM.
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Anti-SCN1B antibody (ab107370) at 1/100
dilution + Mouse Neuro-2a cell line lysates at
35 µg
Predicted band size : 25 kDa
Western blot - SCN1B antibody (ab107370)
ab107370 at 1/50 dilution staining SCN1B in
formalin-fixed and paraffin-embedded Human
brain tissue by Immunohistochemistry,
followed by peroxidase conjugation of the
secondary antibody and DAB detection.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - SCN1B antibody
(ab107370)
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