Product datasheet
Anti-Nicotinic Acetylcholine Receptor beta antibody
[EP2067Y] ab76159
2 Images
Overview
Product name
Anti-Nicotinic Acetylcholine Receptor beta antibody [EP2067Y]
Description
Rabbit monoclonal [EP2067Y] to Nicotinic Acetylcholine Receptor beta
Tested applications
WB, ICC, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in the extracellular region of human Nicotinic
Acetylcholine Receptor beta
Positive control
Human brain lysate.
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2067Y
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab76159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 57 kDa.
ICC
1/100.
1
Application
Flow Cyt
Abreviews
Notes
1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Application notes
Is unsuitable for IHC-P or IP.
Target
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that
affects all subunits and leads to opening of an ion-conducting channel across the plasma
membrane.
Involvement in disease
Defects in CHRNB1 are a cause of congenital myasthenic syndrome slow-channel type
(SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome.
Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial
and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and
ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and
leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is
caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and
prolonged AChR channel opening episodes.
Defects in CHRNB1 are a cause of congenital myasthenic syndrome with acetylcholine receptor
deficiency (ACHRDCMS) [MIM:608931]. ACHRDCMS is a post-synaptic congenital myasthenic
syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive
inheritance.
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1)
subfamily. Beta-1/CHRNB1 sub-subfamily.
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Anti-Nicotinic Acetylcholine Receptor beta antibody [EP2067Y] images
2
Overlay histogram showing SHSY-5Y cells
stained with ab76159 (red line). The cells
were fixed with 80% methanol (5 min) and
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions. The cells were
then incubated with the antibody (ab76159,
1/1000 dilution) for 30 min at 22°C. The
secondary antibody used was Alexa Fluor®
Flow Cytometry - Anti-Nicotinic Acetylcholine
Receptor beta antibody [EP2067Y] (ab76159)
488 goat anti-rabbit IgG (H&L) (ab150077) at
1/2000 dilution for 30 min at 22°C. Isotype
control antibody (black line) was rabbit IgG
(monoclonal) (0.1μg/1x106 cells) used under
the same conditions. Unlabelled sample (blue
line) was also used as a control. Acquisition
of >5,000 events were collected using a
20mW Argon ion laser (488nm) and 525/30
bandpass filter. This antibody gave a positive
result in 4% paraformaldehyde (10 min) fixed
SHSY-5Y cells used under the same
conditions. Please note that Abcam do not
have any data for use of this antibody on nonfixed cells. We welcome any customer
feedback.
Anti-Nicotinic Acetylcholine Receptor beta
antibody [EP2067Y] (ab76159) at 1/1000
dilution + human brain lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 57 kDa
Observed band size : 57 kDa
Western blot - Nicotinic Acetylcholine Receptor
beta antibody [EP2067Y] (ab76159)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
3
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4