Product datasheet
Anti-KCNJ5 antibody ab113699
2 Images
Overview
Product name
Anti-KCNJ5 antibody
Description
Rabbit polyclonal to KCNJ5
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Macaque Monkey,
Gorilla, Chinese Hamster, Orangutan, Elephant
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KCNJ5
.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in Human Testis tissue lysate as well as the following whole
cell lysates: A489; MCF7; A549. It also gave a positive signal in FFPE human pancreas tissue
sections
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab113699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
WB
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa
(predicted molecular weight: 48 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
Target
Function
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are
characterized by a greater tendency to allow potassium to flow into the cell rather than out of it.
Their voltage dependence is regulated by the concentration of extracellular potassium; as
external potassium is raised, the voltage range of the channel opening shifts to more positive
voltages. The inward rectification is mainly due to the blockage of outward current by internal
magnesium. Can be blocked by external barium.
Tissue specificity
Islets, exocrine pancreas and heart.
Involvement in disease
Defects in KCNJ5 are the cause of long QT syndrome type 13 (LQT13) [MIM:613485]. It is a
heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular
arrhythmias. They cause syncope and sudden death in response to excercise or emotional
stress, and can present with a sentinel event of sudden cardiac death in infancy.
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.
Cellular localization
Membrane.
Anti-KCNJ5 antibody images
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All lanes : Anti-KCNJ5 antibody (ab113699)
at 1 µg/ml
Lane 1 : Testis (Human) Tissue Lysate - adult
normal tissue (ab30257)
Lane 2 : A498 (Human Kidney Carcinoma)
Whole Cell Lysate
Lane 3 : MCF7 (Human breast
adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma
epithelial cell line) Whole Cell Lysate
Western blot - Anti-KCNJ5 antibody (ab113699)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 50 kDa
Additional bands at : 120 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 1 minute
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IHC image of KCNJ5 staining in human
pancreas formalin fixed paraffin embedded
tissue section, performed on a Leica BondTM
system using the standard protocol F. The
section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer
(pH6, epitope retrieval solution 1) for 20 mins.
The section was then incubated with
ab113699, 1µg/ml, for 15 mins at room
temperature and detected using an HRP
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-KCNJ5
antibody (ab113699)
conjugated compact polymer system. DAB
was used as the chromogen. The section was
then counterstained with haematoxylin and
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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