Product datasheet
Anti-KCNE2 antibody ab69376
2 Images
Overview
Product name
Anti-KCNE2 antibody
Description
Rabbit polyclonal to KCNE2
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human
KCNE2.Read Abcam's proprietary immunogen policy(Peptide available as ab86641.)
Positive control
This antibody gave a positive signal in mouse pancreas tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab69376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use at an assay dependent concentration. Detects a band of approximately 17
kDa (predicted molecular weight: 14 kDa).
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
Target
1
Function
Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel
complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of
the channel complex. Associated with KCNH2/HERG is proposed to form the rapidly activating
component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2
and/or KCNQ3 and modulate the native M-type current. May associate with KCNQ1/KVLTQ1
and elicit a voltage-independent current. May associate with HCN1 and HCN2 and increase
potassium current.
Tissue specificity
Highly expressed in brain, heart, skeletal muscle, pancreas, placenta, kidney, colon and thymus.
A small but significant expression is found in liver, ovary, testis, prostate, small intestine and
leukocytes. Very low expression, nearly undetectable, in lung and spleen.
Involvement in disease
Defects in KCNE2 are the cause of long QT syndrome type 6 (LQT6) [MIM:613693]. Long QT
syndromes are heart disorders characterized by a prolonged QT interval on the ECG and
polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to
exercise or emotional stress. KCNE2 mutants form channels that open slowly and close rapidly,
thereby diminishing potassium currents.
Defects in KCNE2 are the cause of atrial fibrillation familial type 4 (ATFB4) [MIM:611493]. Atrial
fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of
patients. It is characterized by disorganized atrial electrical activity and ineffective atrial
contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in
palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Sequence similarities
Belongs to the potassium channel KCNE family.
Cellular localization
Membrane.
Anti-KCNE2 antibody images
Anti-KCNE2 antibody (ab69376) at 1 µg/ml +
Pancreas (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Predicted band size : 14 kDa
Observed band size : 17 kDa
Western blot - KCNE2 antibody (ab69376)
2
IHC image of KCNE2 staining in human
normal kidney formalin fixed paraffin
embedded tissue section, performed on a
Leica BondTM system using the standard
protocol F. The section was pre-treated using
heat mediated antigen retrieval with sodium
citrate buffer (pH6, epitope retrieval solution
1) for 20 mins. The section was then
incubated with ab69376, 5µg/ml, for 15 mins
at room temperature and detected using an
HRP conjugated compact polymer system.
Immunohistochemistry (Formalin/PFA-fixed
DAB was used as the chromogen. The
paraffin-embedded sections) - KCNE2 antibody
section was then counterstained with
(ab69376)
haematoxylin and mounted with DPX.
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