Product datasheet
Anti-HRH4 antibody ab97997
1 Image
Overview
Product name
Anti-HRH4 antibody
Description
Rabbit polyclonal to HRH4
Tested applications
WB
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse
HRH4.Read Abcam's proprietary immunogen policy(Peptide available as ab109635.)
Positive control
This antibody gave a positive signal in Raw264.7 and EL4 whole cell lysates as well as the
following tissue lysates: Mouse brain; Mouse bone marrow; Mouse spleen; Mouse thymus.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab97997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa
(predicted molecular weight: 44 kDa).
Target
1
Function
The H4 subclass of histamine receptors could mediate the histamine signals in peripheral
tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of
agonist).
Tissue specificity
Expressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells
of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils.
Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung,
pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph
node.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Anti-HRH4 antibody images
All lanes : Anti-HRH4 antibody (ab97997) at
1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Bone Marrow Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lane 4 : Thymus (Mouse) Tissue Lysate
Lane 5 : RAW 264.7 (Mouse leukaemic
monocyte macrophage cell line) Whole Cell
Lysate
Lane 6 : EL4 (Mouse) Whole Cell Lysate
Western blot - HRH4 antibody (ab97997)
(ab7183)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 68 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 12 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
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