Product datasheet
Anti-HRH3 antibody [EPR5631] ab124732
3 Images
Overview
Product name
Anti-HRH3 antibody [EPR5631]
Description
Rabbit monoclonal [EPR5631] to HRH3
Tested applications
WB, Flow Cyt, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues on the N-terminus in the extracellular domain of
human HRH3 was used as an immunogen.
Positive control
Lysates of: Jurkat, SH-SY5Y, C6, A549, Neuro-2a Jurkat cells
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 2.62 x 10 -10 M
Learn more about KD
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR5631
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab124732 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt
1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an
isotype control with this antibody.
ICC/IF
Application notes
1/100 - 1/250.
Is unsuitable for IHC-P or IP.
Target
Function
The H3 subclass of histamine receptors could mediate the histamine signals in CNS and
peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high
constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of
isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium
mobilization.
Tissue specificity
Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate
nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level
varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the
thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor
expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra,
cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Anti-HRH3 antibody [EPR5631] images
All lanes : Anti-HRH3 antibody [EPR5631]
(ab124732)
Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : C6 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-HRH3 antibody [EPR5631]
(ab124732)
Predicted band size : 49 kDa
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Flow cytometric analysis of Jurkat cells using
ab124732 (red)at 1/100 dilution or a rabbit
IgG (negative) (green)
Flow Cytometry - Anti-HRH3 antibody [EPR5631]
(ab124732)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-HRH3 antibody [EPR5631](ab124732)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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