Product datasheet
Anti-PRR7 antibody [TRAP3/10] ab91499
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Overview
Product name
Anti-PRR7 antibody [TRAP3/10]
Description
Mouse monoclonal [TRAP3/10] to PRR7
Tested applications
WB, ICC
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant fragment, corresponding to C terminal half of the intracellular domain (amino acids
126-253) of Human PRR7
Positive control
Murine brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Purity
Protein A purified
Purification notes
Purified from ascites. Purity: > 95% (by SDS-PAGE).
Clonality
Monoclonal
Clone number
TRAP3/10
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab91499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
ICC
Application notes
ICC: Use at a concentration of 10 µg/ml.
Note: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1%
Triton-X100
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Cellular localization
Membrane. Cell junction > synapse > postsynaptic cell membrane. Enriched in post-synaptic
plasma membrane and postsynaptic densities (PSD). Accumulates in spines along with
synapse maturation and colocalizes with DLG4 in a punctate pattern.
Anti-PRR7 antibody [TRAP3/10] images
Immunofluorescence showing ab91499 at 10
µl/ml staining HEK-293 cells cotransfected
with PRR7 (red) and GFP-PSD-95 (green).
DNA visualized by DAPI (blue).
Immunocytochemistry/ Immunofluorescence PRR7 antibody [TRAP3/10] (ab91499)
Anti-PRR7 antibody [TRAP3/10] (ab91499) at
1 µg/ml + Murine brain lysate
Predicted band size : 31 kDa
Western blot - PRR7 antibody [TRAP3/10]
(ab91499)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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