Anti-PRR7 antibody [TRAP3/10] ab91499 Product datasheet 2 Images Overview

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Product datasheet
Anti-PRR7 antibody [TRAP3/10] ab91499
2 Images
Overview
Product name
Anti-PRR7 antibody [TRAP3/10]
Description
Mouse monoclonal [TRAP3/10] to PRR7
Tested applications
WB, ICC
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant fragment, corresponding to C terminal half of the intracellular domain (amino acids
126-253) of Human PRR7
Positive control
Murine brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Purity
Protein A purified
Purification notes
Purified from ascites. Purity: > 95% (by SDS-PAGE).
Clonality
Monoclonal
Clone number
TRAP3/10
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab91499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
ICC
Application notes
ICC: Use at a concentration of 10 µg/ml.
Note: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1%
Triton-X100
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Cellular localization
Membrane. Cell junction > synapse > postsynaptic cell membrane. Enriched in post-synaptic
plasma membrane and postsynaptic densities (PSD). Accumulates in spines along with
synapse maturation and colocalizes with DLG4 in a punctate pattern.
Anti-PRR7 antibody [TRAP3/10] images
Immunofluorescence showing ab91499 at 10
µl/ml staining HEK-293 cells cotransfected
with PRR7 (red) and GFP-PSD-95 (green).
DNA visualized by DAPI (blue).
Immunocytochemistry/ Immunofluorescence PRR7 antibody [TRAP3/10] (ab91499)
Anti-PRR7 antibody [TRAP3/10] (ab91499) at
1 µg/ml + Murine brain lysate
Predicted band size : 31 kDa
Western blot - PRR7 antibody [TRAP3/10]
(ab91499)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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