Product datasheet
Anti-Phosphotyrosine antibody ab17302
1 Abreviews 1 Image
Overview
Product name
Anti-Phosphotyrosine antibody
Description
Rabbit polyclonal to Phosphotyrosine
Specificity
Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
Tested applications
ELISA, WB, IP
Species reactivity
Reacts with: Species independent
Immunogen
Affinity purified native tyrosine phosphorylated proteins (Human).
Positive control
A431 Cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at 20°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 3mM Sodium Azide
Constituents: PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab17302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
1/5000 - 1/10000.
WB
1/1000 - 1/2000.
IP
Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.
Target
1
Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of
signal transduction during normal mitogenesis, cell cycle progression and oncogenic
transformation, its role in other areas such as differentiation and gap junction communication, is
a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated
tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins
and the biochemical pathways in which they function.
Anti-Phosphotyrosine antibody images
All lanes : Anti-Phosphotyrosine antibody
(ab17302)
Lane 1 : Untreated parotid acinar cells
Lane 2 : Parotid acinar cells with 50µM
etoposide for 2 hours
Lane 3 : Parotid acinar cells with 5mM H2O2
for 30 min
Lysates/proteins at 50 µg per lane.
Western blot - Phosphotyrosine antibody
Secondary
(ab17302)
HRP-conjugated Donkey anti-Rabbit IgG at
1/10000 dilution
Performed under reducing conditions.
Exposure time : 1 minute
This image is courtesy of an anonymous
Abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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