Product datasheet
Anti-Phosphoserine/threonine/tyrosine antibody
[SPM101] ab15556
6 Abreviews 8 References 3 Images
Overview
Product name
Anti-Phosphoserine/threonine/tyrosine antibody [SPM101]
Description
Mouse monoclonal [SPM101] to Phosphoserine/threonine/tyrosine
Specificity
ab15556 recognises serine, threonine, and tyrosine phosphorylated proteins. It may cross-react
with other phosphorylated amino acids.
Tested applications
IHC - Wholemount, Functional Studies, ICC/IF, Flow Cyt, ELISA, WB, IP, IHC-P, IHC-Fr
Species reactivity
Reacts with: Species independent
Immunogen
Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers.
Positive control
Breast carcinoma
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Purity
Protein G purified
Purification notes
Purified from ascites fluid by Protein G.
Clonality
Monoclonal
Clone number
SPM101
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab15556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC - Wholemount
1/200.
Functional Studies
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
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Application
Abreviews
Notes
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
Flow Cyt
an isotype control with this antibody.
ELISA
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Milk derived blocking solutions reportedly
contain phosphoproteins that may inhibit phosphoamino acid antibody binding
and therefore should be avoided.
IP
Use at 2 µg/mg of lysate.
IHC-P
1/20. Boil tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling
at RT for 20 min.
IHC-Fr
Use at an assay dependent concentration. PubMed: 20421451
Target
Relevance
Protein phosphorylation provides a signalling system that can be thought of as a kind of protein
on/off switch for many cellular signalling pathways. Phosphorylation is observed on serine,
threonine, tyrosine and histidine residues. Cellular networks underlying phosphorylation can be
very complex and often occurs on multiple distinct sites on a given protein. Phospho-specific
antibodies are becoming critical reagents both for basic research and for clinical diagnosis.
Anti-Phosphoserine/threonine/tyrosine antibody [SPM101] images
Human breast carcinoma stained with
ab15556. Immunohistochemistry (FFPEsections).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) Phosphoserine/threonine/tyrosine antibody
[SPM101] (ab15556)
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Overlay histogram showing MCF7 cells
stained with ab15556 (red line). The cells
were fixed with 4% paraformaldehyde (10
min) and then permeabilized with 0.1% PBSTween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions followed by the
Flow Cytometry -
antibody (ab15556, 1µg/1x106 cells) for 30
Phosphoserine/threonine/tyrosine antibody
min at 22ºC. The secondary antibody used
[SPM101] (ab15556)
was DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in MCF7
cells fixed with 80% methanol (5
min)/permeabilized in 0.1% PBS-Tween used
under the same conditions.
Immunohistochemical analysis of mouse
cardiomyocytes, staining
Phosphoserine/threonine/tyrosine with
ab15556.
IHC - Wholemount - AntiPhosphoserine/threonine/tyrosine antibody
Samples were incubated with primary
[SPM101] (ab15556)
antibody (1/200 in diluent) for 1 hour. An
This image is courtesy of an anonymous Abreview
AlexaFluor®488-conjugated goat anti-mouse
polyclonal IgG (1/400) was used as the
secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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