Product datasheet
Anti-RITA antibody ab122531
1 Image
Overview
Product name
Anti-RITA antibody
Description
Rabbit polyclonal to RITA
Tested applications
IHC-P
Species reactivity
Reacts with: Human
Immunogen
antigen sequence, corresponding to amino acids 167-254 of Human RITA (UniProt ID:
Q96K30).
Run BLAST with
Positive control
Run BLAST with
Human rectum tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab122531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol. Use buffer at pH 6 for antigen
retrieval.
Target
1
Function
Tubulin-binding protein that acts as a negative regulator of Notch signaling pathway. Shuttles
between the cytoplasm and the nucleus and mediates the nuclear export of RBPJ/RBPSUH,
thereby preventing the interaction between RBPJ/RBPSUH and NICD product of Notch proteins
(Notch intracellular domain), leading to down-regulate Notch-mediated transcription. May play a
role in neurogenesis.
Sequence similarities
Belongs to the RITA family.
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. Shuttles rapidly between the
cytoplasm and the nucleus. The function of centrosome localization is still unclear.
Anti-RITA antibody images
Immunohistochemical staining of RITA in
paraffin embedded Human rectum tissue by
Immunohistochemistry, using ab122531 at a
1/75 dilution. The image shows cytoplasmic
and membranous positivity in glandular cells.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-RITA antibody
(ab122531)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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