Product datasheet
Anti-Cannabinoid Receptor I antibody ab3559
5 Abreviews 2 References 4 Images
Overview
Product name
Anti-Cannabinoid Receptor I antibody
Description
Rabbit polyclonal to Cannabinoid Receptor I
Tested applications
ICC, WB, IP, IHC-P, IHC-Fr, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Cat, Human, Ferret, Fish, Monkey, Non Human
Primates, Amphibians, Goldfish, Salamander
Immunogen
Fusion protein corresponding to Rat Cannabinoid Receptor I aa 1-77 (N terminal).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab3559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC
1/1000.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
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Application
Abreviews
Notes
Flow Cyt
Use at an assay dependent concentration. See Abreview.
Target
Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a
receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have
altered ligand binding.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Anti-Cannabinoid Receptor I antibody images
Lane 1 : Anti-Cannabinoid Receptor I
antibody (ab3559) at 1/500 dilution
Lanes 2 - 3 : Anti-Cannabinoid Receptor I
antibody (ab3559) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Western blot - Anti-Cannabinoid Receptor I
antibody (ab3559)
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) analysis of rat
hippocampus tissue sections labeling
Cannabinoid Receptor I with ab3559.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Cannabinoid
Receptor I antibody (ab3559)
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Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) analysis
of transverse spinal cord E6 chick
embryo tissue sections labeling Cannabinoid
Receptor I with ab3559.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Cannabinoid
Receptor I antibody (ab3559)
ab3559 staining the Cannabinoid Receptor 1
in Human synovial fibroblasts by Flow
Cytometry. Cells were fixed with 1.5%
formaldehyde, pelleted and
permeabilized with 100% ice cold
methanol for at least 10 minutes. Gating was
done on synovial fibroblasts. The sample was
incubated with the primary antibody, dilution
1/100 (1% BSA in PBS), for 15 minutes. An
Abcam PE-conjugated donkey polyclonal to
rabbit IgG ( ab7007), dilution 1/200, was used
Flow Cytometry - Cannabinoid Receptor I antibody
as secondary antibody. The red peak shows
(ab3559)
staining with ab3559.
This image is courtesy of an Abreview submitted by
Dr Torsten Lowin
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Terms and conditions
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