Product datasheet
Anti-Phosphoserine antibody [3C171] ab17465
1 Abreviews 4 References 2 Images
Overview
Product name
Anti-Phosphoserine antibody [3C171]
Description
Mouse monoclonal [3C171] to Phosphoserine
Specificity
Reacts against phosphorylated serine both as free amino acid or when conjugated to carriers
such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine,
phosphotyrosine, AMP or ATP.
Tested applications
ELISA, WB, ICC/IF
Species reactivity
Reacts with: Species independent
Immunogen
Phosphoserine conjugated to KLH.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide, 0.01% Thimerosal.
Constituents: 40% Glycerol, PBS, 15mg/ml BSA. pH 7.6
Purity
Ascites
Clonality
Monoclonal
Clone number
3C171
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab17465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
1/500.
WB
1/250. (colorimetric). Do not use milk as a blocking agent or in diluents, as milk
casein is phosphorylated at several serine residues. BSA is recommended
instead.
ICC/IF
Use a concentration of 1 µg/ml.
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Target
Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various
external stimuli can have profound effects on cellular signal transduction, apoptosis and
carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the
phosphospecific amino acid, are important tools to explore the activation of serine, threonine or
tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human
disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are
recognized as a major new drug target family.
Anti-Phosphoserine antibody [3C171] images
ICC/IF image of ab17465 stained MCF-7
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab17465, 1µg/ml) overnight
Immunocytochemistry/ ImmunofluorescencePhosphoserine antibody [3C171](ab17465)
at +4°C. The secondary antibody (green) was
Alexa Fluor® 488 goat anti-mouse IgG (H+L)
used at a 1/1000 dilution for 1h. Alexa Fluor®
594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
Western blot of canine brain hippocampus
homogenates (10 ug loaded per lane for 3
different samples) separated on SDS-PAGE
and blotted with ab17465.
Western blot - Phosphoserine antibody [3C171]
(ab17465)
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