Product datasheet Anti-SOX21 antibody ab24604 3 Abreviews 1 Image Overview Product name Anti-SOX21 antibody Description Rabbit polyclonal to SOX21 Tested applications IHC-P Species reactivity Reacts with: Rat Predicted to work with: Mouse, Chicken, Human, Zebrafish Immunogen Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SOX21. Read Abcam's proprietary immunogen policy (Peptide available as ab26974.) Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle. Storage buffer Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Purity Immunogen affinity purified Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab24604 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application IHC-P Abreviews Notes 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Application notes Is unsuitable for IHC-Fr. Target Function May play a role as an activator of transcription of OPRM1. 1 Sequence similarities Contains 1 HMG box DNA-binding domain. Cellular localization Nucleus. Anti-SOX21 antibody images Immunohistochemistry (Formaldehyde/PFAfixed paraffin-embedded sections) using SOX21 antibody (ab24604)on Rat Tissue sections (adult brain lateral ventricle subventricular zone). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT°C. ab24604 used at 1/300 incubated for 16 hours. Secondary Antibody: biotin conjugated anti rabbit IgG (1/300). NB: Immunohistochemistry (Formalin/PFA-fixed Sox21 protein expression showin in this paraffin-embedded sections) - SOX21 antibody image is in concurrs with published mRNA (ab24604) positivity in the subventricular zone and rostro- Carl Hobbs, KCL, CARD, London, UK migratory stream in the adult rat. Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2