Product datasheet
Anti-Mitofusin 1 antibody [11E91H12] ab126575
2 Abreviews 1 References 3 Images
Overview
Product name
Anti-Mitofusin 1 antibody [11E91H12]
Description
Mouse monoclonal [11E91H12] to Mitofusin 1
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human
Immunogen
Recombinant Mouse Mitofusin 1 lacking the transmembrane region (Q8IWA4).
Positive control
H4IIE Rat cell lysate, NIH 3T3 Mouse cell lysate.
General notes
Product was previously marketed under the MitoSciences sub-brand.
Alternative versions available:
Anti-Mitofusin 1 antibody (HRP) [11E91H12] (ab198410)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HBS
Purity
Ammonium Sulphate Precipitation
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by
ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE.
Clonality
Monoclonal
Clone number
11E91H12
Isotype
IgG2b
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab126575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
Target
Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of
mitochondria occurs in many cell types and constitutes an important step in mitochondria
morphology, which is balanced between fusion and fission. MFN1 acts independently of the
cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Tissue specificity
Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be
overexpressed in some tumors, such as lung cancers.
Sequence similarities
Belongs to the mitofusin family.
Post-translational
modifications
Ubiquitinated by MARCH5.
Cellular localization
Cytoplasm and Mitochondrion outer membrane.
Anti-Mitofusin 1 antibody [11E91H12] images
All lanes : Anti-Mitofusin 1 antibody
[11E91H12] (ab126575) at 5 µg/ml
Lane 1 : H4IIE Rat cell lysate
Lane 2 : NIH 3T3 Mouse cell lysate
Lane 3 : Mouse embryonic fibroblast cell line,
wild-type
Lane 4 : Mouse embryonic fibroblast cell line,
Mitofusin 1-deficient
Western blot - Anti-Mitofusin 1 antibody
Lane 5 : Mouse embryonic fibroblast cell line,
[11E91H12] (ab126575)
Mitofusin 2-deficient
Lysates/proteins at 40 µg per lane.
Secondary
Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size : 84 kDa
2
All lanes : Anti-Mitofusin 1 antibody
[11E91H12] (ab126575) at 5 µg/ml
Lane 1 : H4IIE Rat cell lysate
Lane 2 : Mouse embryonic fibroblast cell line,
wild-type
Lane 3 : Mouse embryonic fibroblast cell line,
Mitofusin 1-deficient
Western blot - Anti-Mitofusin 1 antibody
Lane 4 : Mouse embryonic fibroblast cell line,
[11E91H12] (ab126575)
Mitofusin 2-deficient
Lysates/proteins at 40 µg per lane.
Secondary
Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size : 84 kDa
ab126575 at 5 µg/ml (2 hrs) staining Mitofusin
1 in paraformaldehyde fixed (4%, 20 min) and
Triton X-100 permeabilized (0.1%, 15min)
Mouse embryonic fibroblast cells by
Immunofluorescence. Secondary: 1/2000
AlexaFluor® 488 goat anti-mouse IgG (H+L).
DAPI was used to stain the cell nuclei (blue).
Abcam Blocking Buffer (ab126587) was used
Immunocytochemistry/ Immunofluorescence -
throughout.
Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
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